LI-COR Biosciences - IRDye 800CW EGF Optical Probe

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Molecular Imaging Seminar Series from Baylor College of Medicine and the University of Nebraska Medical Center.

Why Target EGFR
IRDye^® 800CW EGF Optical Probe is a near-infrared (NIR) labeled recombinant human epidermal growth factor (EGF). Epidermal growth factor receptor (EGFR) is one of a family of receptor tyrosine kinases found on the surface of epithelial cells, to which EGF binds. Many types of cancer cells have abnormally high EGFR levels on the cell surface (Table 1). In vivo assays of EGF binding have proven effective in monitoring specific solid tumor models that exhibit over-expression of EGFR^2-6. EGF conjugated to LI-COR's IRDye 800CW NIR fluorophore provides a versatile probe that can be used for in vitro and in vivo assays, as well as whole organ and tissue section analysis.

Advantages of Near-Infrared Optical Probes

Very high signal-to-noise ratio.
Low NIR autofluorescence from tissues, cells, biological materials, and drug compounds.
IRDye 800CW absorption/emission near 800 nm matches a NIR absorption minima for bodily fluids and tissues, resulting in excellent tissue penetration.

Probe Specificity
In-Cell Western assays on the LI-COR Odyssey^® and Aerius^® Infrared Imaging Systems are used to determine binding specificity of the probe in vitro for various cell lines (Figure 1). IRDye 800CW EGF has been characterized in A431 (squamous epithelial carcinoma, Figure 2), and PC3M-LN4 and 22Rv1 (human prostate adenocarcinoma) cell lines that over-express EGFR.

Figure 1 - Two different cell lines known to over-express the EGFR were characterized by evaluating:

binding of IRDye 800 CW EGF;
IRDye 800CW only;
competition assay using unlabeled EGF to compete for EGFR sites

Figure 2 - Two Athymic Nu/Nu tumor-bearing mice were injected with either 0.9% saline (left) or 1 nmol IRDye 800CW EGF (right) 72 hours prior to imaging with the LI-COR Odyssey Infrared Imaging System

Specifications

Description: Recombinant EGF polypeptide containing 54 amino acid residues (molecular weight = 6.2 kDa) conjugated to the LI-COR IRDye 800CW NIR fluorophore.

800CW PBS Chart

Properties (in 1X PBS):
• Absorption maximum: 774 nm
• Emission maximum: 789 nm

Ordering Information

Part #	Description	Unit/Size	Downloads	Order Online
926-08446	IRDye 800CW EGF Optical Probe 20 nanomoles (approximately 10 injections)		description msds pack insert	$ 325.00
928-38040	IRDye 800CW Protein Labeling Kit - High Molecular Weight Contents: 3x IRDye 800CW reactive dye vials (0.1 mg) 1x 0.5 ml 1M potassium phosphate 1x 25 ml 1X PBS 1x 0.5 ml ultra-pure water 3x desalting spin columns	1 kit	description msds pack insert	$ 375.00
928-38042	IRDye 800CW Protein Labeling Kit - Low Molecular Weight Contents: 3x IRDye 800CW reactive dye vials (0.5 mg) 1x 0.5 ml 1M potassium phosphate 1x 25 ml 1X PBS 1x 0.5 ml ultra-pure water 3x desalting spin columns	1 kit	description msds pack insert	$ 425.00
928-38044	IRDye 800CW Protein Labeling Kit - Microscale Contents: 3x IRDye 800CW reactive dye vials (0.1 mg) 1x 0.5 ml 1M potassium phosphate 1x 25 ml 1X PBS 1x 0.5 ml ultra-pure water 3x desalting spin columns	1 kit	description msds pack insert	$ 325.00
929-70020	IRDye 800CW NHS ESTER 0.5mg	0.5mg	description msds pack insert	$ 130.00
929-70021	IRDye 800CW NHS ESTER 5.0mg	5.0mg	description msds pack insert	$ 925.00
9201-01	Odyssey Infrared Imaging System		description	Inquire
9201-MP	Odyssey MousePOD		description	$ 14,100.00

For research purposes only. Not for diagnositc or therapeutic use. Not for use in humans. LI-COR IRDye 800 and other LI-COR reagent products are covered by U.S. patents, foreign equivalents, and patents pending.

Shipping information: U.S.A.: Same-day shipping (Federal Express overnight) available on orders received before 12:00 noon CST. Canada: Allow 3-5 days for shipments. Germany & UK: Contact LI-COR GmbH. International: Contact a LI-COR distributor in your area. All shipments subject to availability.

References

1. Hong, W. and A. Ullrich. Oncology Biotherapeutics. Vol. 1, No. 1, p. 5, 2000.

2. Spano, J., Fagard, R., Soria, J., Rixe, O., Khayat D., and G. Milano. Epidermal growth factor receptor signaling in colorectal cancer: preclinical data and therapeutic perspectives. Annals of Oncology, 16: 189-194, 2005.

3. Onn, A., Correa, A., Gilcrease, M., Isobe, T., Massarelli, E., Bucana, C., O'Reilly, M., Hong, W., Fidler, I., Putnam, J., and R. Herbst. Synchronous overexpression of epidermal growth factor receptor and HER2-neu protein is a predictor of poor out- come in patients with stage I non-small cell lung cancer. Clinical Cancer Research, 10: 136-143, 2004.

4. Sokolov, K., Follen, M., Aaron, J., Pavlova, I., Malpica, A., Lotan, R., and R. Richards-Kortum. Real- time vital optical imaging of precancer using anti- epidermal growth factor receptor antibodies conju- gated to gold nanoparticles. Cancer Research, 63: 1999-2004, 2003.

5. Ke, S., Wen, X., Gurfinkel, M., Charnsangavej, C., Wallace, S., Sevick-Muraca, E., and C. Li. Near- infrared optical imaging of epidermal growth factor receptor in breast cancer xenografts. Cancer Research, 63: 7870-7875, 2003.

6. Chakravarti, A., Delaney, M., Noll, E., Black, P., Loeffler, J., Muzikansky, A., and N. Dyson. Prognostic and pathologic significance of quantita- tive protein expression profiling in human gliomas. Clinical Cancer Research, 7: 2387-2395, 2001.

IRDye is a registered trademark of LI-COR Inc.

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