Cell biologists now have a new "color" for multi-color imaging when near-infrared (NIR) labeled antibodies are imaged using a new filter set designed exclusively for LI-COR by Chroma Technology Corporation - a world leader in filter design and manufacturing.

The Infrared Advantage:

• Very low autofluorescence for exceptionally low backgrounds.
• Lower energy NIR excitation wavelength causes less sample damage than visible wavelengths.
• No spectral overlap with visible dyes.

Very Low Autofluorescence

Primary and secondary antibodies conjugated with
LI-COR's IRDye® 800 Infrared Dye produce images with very low backgrounds and high signal sensitivity. IRDye® 800 infrared dye is part of LI-COR's exclusive family of NIR dyes that fluoresce at approximately 800 nm. Since most biomolecules have very low autofluorescence in the NIR region, IRDye® 800 infrared dye provides a level of performance not available with visible dyes. Bright, clear images with extremely clean backgrounds and excellent sensitivity like those shown below are typical with LI-COR's IRDyes.

Immunocytochemistry
With IRDye® 800 Infrared Dye Antibodies

	Three-color image of a mitotic cell. NIH 3T3 cells were stained with anti-tubulin primary and IRDye® 800 infrared dye secondary antibody (shown in red), human CREST serum to show kinetochores (Texas Red™, green (false color)), and Hoechst for chromosomal DNA (blue).
	Staining of duplicated centrosomes. Condensed chromosomes are stained with DAPI (blue). The two centrosomes (red dots) are stained with a primary antibody against pericentrin (a centrosomal component) and IRDye® 800 infrared dye secondary antibody.

No Spectral Overlap With
Visible Dyes

The emission maxima of IRDye® 800 infrared dye is at 806 nm.

Since the emission of IRDye® 800 infrared dye is separated by more than 100 nm from most commonly used dyes (Cy5 emits at 670 nm), there is no spectral operlap or bleed-through between channels.

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