Biotechnology
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DNA Sequencing and Genetic Analysis
LI-COR Instruments for IR Applications
In DNA sequencing applications, LI-COR instrumentation has become renown for very long reads with superior accuracy. Infrared detection is the key enabling technology that extends readlength and improves accuracy. Infrared technology has also increased genotyping accuracy, while reducing the cost per genotype due to lower reagent usage.
Independent Detection
The 4300 DNA Analysis System features LI-COR's unique, two channel detection that eliminates errors due to fluorescence overlap. The two IR dyes used in this system were carefully designed so that their fluorescence is separated by over 100 nm. Two separate sets of optically tuned lasers and detectors independently measure fluorescence signals from the IR dyes.
With no signal crossover due to fluorescence overlap, band are easier to discriminate on DNA sequencing images and read lengths extend to over 1000 bases per sample with 99% accuracy (model 4300L). Band detection with no signal crossover also results in high quality genotyping images, similar to an autoradiogram, that are easily and accurately analyzed by genotyping software.
High Sensitivity and Wide Dynamic Range
The TIFF images generated by the 4300 System use more than 65,000 grayscales per pixel (16-bit) to display the wide dynamic range of the infrared detection system. Having wide dynamic range reduces rework in the lab because underloaded or overloaded samples are accurately detected.
Two Separate Detectors
As samples pass in front of the scanning laser/microscope, two solid-state avalanche photodiodes detect fluorescence. Optical filtering assures that each detector measures fluorescence from only one of the infrared dyes.
Two Diode Lasers
Diode lasers, similar to those in compact disk players, provide high output with low power consumption.
High Reliability
The high reliability of solid-state lasers and detectors allows LI-COR to warranty the laser/microscope for three years.
Two Real Time Images
A separate image for each IR dye is collected in real time and displayed in e-Seq software, along with standard fluorescence curves.
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