IRDye® 800RS NHS Ester

IRDye 800 RS is the dye of choice for nucleic acid applications. The NHS ester reactive group provides the functionality for labeling primary and secondary amino groups. Nucleic acids labeled with this dye are easily purified with reverse-phase chromatography. The dye has good water solubility, but low salt tolerance, and is more hydrophobic than IRDye 800CW. This dye is not recommended for most protein applications.

800RS dye structure

chart

Absorbance Spectrum | Emission Spectrum | Combination Graph

Solvent
Extinction Coefficient
M-1cm-1
Abs Max
nm
Em Max
nm
Stokes Shift
nm
Methanol
300,000
770
786
16
Water
200,000
767
786
19
1X PBS
200,000
767
786
19
PBS:Methanol
270,000
770
786
16
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IRDye NHS Esters

   

 

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800 | 1024 | max
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