LI-COR Biosciences - Biotechnology Product Line

NIR Fluorescence vs. Chemiluminescent Detection

The enzymatic chemiluminescent reaction used to produce light is dynamic, constantly changing over time. This time dependence compromises quantification and accuracy. Near infrared fluorescence detection can be compared to a light bulb. All IRDye-labeled antibodies bound to proteins on a Western blot fluoresce at once when excited, making quantification more accurate.

Quantitative Westerns

Chemiluminescence Linear Range: 250-fold

Odyssey Linear Range: >4000-fold

The wide linear range of the Odyssey system makes it easier to detect weak bands when scanning uncharacterized proteins.

Multiplexed Infrared Detection

Low amounts of proteins are accurately quantified when using a ratiometric approach made possible by multiplexed infrared detection on the Odyssey System.


Click to download a paper comparing Western Blot methods for protein analysis	Click to download Image Analysis article on Improving Quantification Accuracy for Western Blots

Recently Published

A comparison of methods for the analysis of low abundance proteins in desmoid tumor cells. Picariello, L., et al. Anal. Biochem. 354: 205-212.

NEW! PDF Listing of Recent Articles featuring Odyssey Data, click here to download. (

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Technical Resources Library

Our Technical Resources Library offers researchers current protocols and additional information on how to use the Odyssey System in your lab.