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Applications for the 4300 Analysis System
AFLP® Analysis
Application Overview
AFLP® Analysis is an application where patterns of genomic restriction fragments can be used to find polymorphisms.
AFLP® Analysis is commonly used for biodiversity or mapping research. AFLP® Analysis does not require knowledge of genomic sequence of the target organism.
Click here to download the AFLP brochure
Advantages
The Infrared Advantage
The 4300 DNA Analyzer turns your samples into data by scanning 96-lane gels during electrophoresis. The two-color laser scanning system expands throughput to 192 lanes per gel by producing a separate gel image for each IRDye® infrared dye. Two sets of lasers and detectors eliminate errors due to fluorescence overlap by detecting the widely separated (100 nm) fluorescence from two different IRDye® infrared dyes.
True gel images of the highest quality are generated because infrared fluorescence detection offers higher sensitivity and inherently lower background due to the lack of infrared fluorescence from most biomolecules. With much wider dynamic range than film, strong and weak bands are accurately detected on the same gel. Bands up to 1000 bp can be resolved since fluorescence is detected as samples move through the gel, rather than after electrophoresis is complete.
True Gel Images
Superior Results with True Gel Images
LI-COR's autoradiogram image format has set the standard as the most easily interpreted, accurate data format for AFLP® analysis. True gel images are easier to interpret than intensity peaks and the familiar format lets you apply all the image interpretation methods you already know.
Like autoradiogram images, LI-COR images allow all samples to be visualized simultaneously for more accurate comparison.
Technical Resources
Protocols:
Webinars and Video Tutorials:
PubMed
High-Throughput AFLP® Analysis Using Infrared Dye-Labeled Primers and an Automated DNA Sequencer.
A.A. Myburg, D.L. Remington, D.M. O'Malley, R.R. Sederoff, and R.W. Whetton.
BioTechniques 2001, 30(2): 348-357.
