Applications for the 4300 Analysis System
Microsatellite genotyping is an application where variations in repeat sequence lengths can be used in population genetics, determination of lineage, and proximity to genes of interest.
Microsatellites are commonly used for biodiversity, forensic, mapping, and association analysis research. Microsatellite analysis requires knowledge of microsatellite regions for the target organism.
Click here to download Microsatellite brochure.
Automated with SagaGT Software - from data collection to allele scoring.
Accurate Analysis - unique allele fingerprinting methods recognize even with di-nucleotide repeats.
High Throughput - 96-lane reloadable (3x) gels.
Mendelian error checking - plus quality values on every allele.
High Sensitivity - infrared detection.
Efficiency through Automation:
Using the 4300 DNA Analyzer and SAGAGT software, entire gene discovery projects can be finished in the time it used to take just to collect the data. SAGAGT eliminates traditional genotyping bottlenecks by analyzing gels in just minutes with no user interaction. Lane finding, location of sizing standards, calibration of band sizes and allele scoring are all automated. SAGAGT also automates data collection, data transfer, and includes an Oracle® database for complete project management.
Runs are started from SAGAGT software, after which the instrument takes over. The 4300 contains a mini-server that controls electrophoresis and stores gel data on its internal hard disk. The computer is not needed during electrophoresis and can be used to analyze gel images. After the run, image data are automatically transferred to SAGAGT and analysis is started.
For labs with networks, the 4300 System provides secure communications that enable a new level of collaboration on projects. If your networks are connected to the Internet, gel runs and SAGAGT projects can be accessed through the Internet, enabling collaboration on an international scale.
LI-COR's exclusive infrared technology provides higher dynamic range for genotyping research. A proven reputation for unparalleled accuracy and reliability results from the use of solid-state lasers and detectors.
The 4300 System features a two channel detection system that uses two separate lasers and detectors to eliminate errors due to fluorescence overlap. Fluorescence of the two IRDye® infrared dyes is separated by 100 nm.
High Sensitivity and Wide Dynamic Range
Infrared detection provides a key advantage due to the high sensitivity and low background fluorescence. Infrared detection reduces the cost per genotype, since fewer reagents are required for detection.
During electrophoresis, two TIFF images are collected that accurately represent the wide range of band intensities that can be detected by the wide dynamic range of the 4300 System. True gel images promote a smooth transition from manual methods.
SagaGT V. 3.3 Microsatellite Analysis Tutorial Guide
SagaGT V. 3.3 Microsatellite Analysis Users Guide
Users guide for V 3.3 of SagaGT
Webinars and Video Tutorials:
Genomics Education Matching Funds Informative Webinar - Jackie Potts
Genetic Linkage Analysis Using Pooled DNA and Infrared Detection of Tailed STRP Primer Patterns.
William S. Oetting, Scott C. Wildenberg and Richard A. King.
SPIE vol. 2680, 226-234.
Linkage Analysis with Multiplex Short Tandem Repeat Polymorphisms Using Infrared Fluorescence and M13 Tailed Primers.
W. S. Oetting, et. al.
Genomics, 1995, 30, 450-458.
Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli.
Lorraine P. Yomano, Robert K. Scopes, L.O. Ingram.
Journal of Bacteriology, July 1993, p. 3926-3933
To see other publications on Microsatellites click here.