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ODYSSEY® Fc IMAGER
Applications for the
Odyssey Fc Imaging System
GEL DOCUMENTATION
Application Overview
Gel Documentation
After electrophoretic separation of protein or nucleic acid samples, gels may be stained and imaged with the Odyssey Fc Imager.
IRDye® Blue (a colloidal Coomassie stain) is commonly used to stain and view polyacrylamide protein gels. Coomassie is a strong IR fluorophore, and can be imaged at 700 nm with the Odyssey® Fc System to achieve superior sensitivity.
Syto® 60, a near-infrared fluorescent DNA stain, can be imaged at 700 nm – and is more sensitive than ethidium bromide. Protocols for Syto 60 gel documentation and band excision can be found at www.licor.com/syto60.
[ABOVE] Purified fetuin protein was serially diluted (9.1 – 0.5 µg) and separated on a 10% gel. The gel was stained with IRDye Blue and imaged with Odyssey Fc in the 700 nm channel. Fetuin resolved as a doublet (2 min acquisition).
[ABOVE] DNA samples (50 bp or 1 kb ladder) were pre-incubated with Syto 60 prior to gel loading. Samples were separated on a 1.2% agarose gel at 80V for 1 h. For each ladder, amounts loaded were 1, 0.5, 0.25, and 0.125 µg (left to right). Gel was imaged with Odyssey Fc in 700 nm channel (2 min acquisition).
