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Applications for the
Odyssey Fc Imaging System
[ABOVE] Purified fetuin protein was serially diluted (9.1 – 0.5 µg) and separated on a 10% gel. The gel was stained with IRDye Blue and imaged with Odyssey Fc in the 700 nm channel. Fetuin resolved as a doublet (2 min acquisition).
After electrophoretic separation of protein samples, gels may be stained and imaged with the Odyssey® Fc Imager.
IRDye® Blue (a colloidal Coomassie stain, P/N 928-40002) is commonly used to stain and view polyacrylamide protein gels. Coomassie is a strong IR fluorophore, and can be imaged at 700 nm with the Odyssey Fc System to achieve superior sensitivity.