- Coomassie Gels
- DNA Staining
- ELISA/FLISA
- EMSA/Gel Shift Assay
- Glycoprotein Detection
- In-Cell Western™ Assay
- In-Gel Western Assay
- Microwestern
- Northern Blot
- On-Cell Western Assay
- Protease Assay
- Protein Array
- Quantitative Western Blot
- Reporter Gene Assay
- Reverse Phase (Lysate) Array
- RNAi Screens
- Small Animal Imaging
- Southern Blot
- Tissue Section Imaging
- Transcription Factor Assay
APPLICATIONS for the
ODYSSEY® IMAGER
Applications for the Odyssey Imaging System
COOMASSIE® GELS
Application Overview
Figure 1. Unstained protein standards were loaded across a 10%SDS-PAGE gel, separated by electrophoresis and stained with Coomassie® Blue. The gel was imaged with the Odyssey Infrared Imaging System in the 700nm channel.
Coomassie® is a registered trade trademark of ICI plc.
IRDye® Blue Protein Stain is a strong 700 nm fluorophore, and its fluorescence is induced upon protein binding.
Can be used for sensitive, quantitative protein detection in gels, with a linear range from ~10 ng to 20 mg1.
More sensitive than white-light imaging of Coomassie staining1-2.
IRDye Blue or Coomassie-stained SDS-PAGE gels can be imaged on the Odyssey Imager with comparable sensitivity to Sypro Ruby2, but at a lower cost.
[ABOVE] Figure 2. Data courtesy of Dr. G. Sarath, University of Nebraska
[ABOVE] Figure 3. Mouse liver membrane and soluble proteins were separated on 2D gels, then stained with Sypro Ruby followed by Coomassie. Image analysis indicates differences in average SNR (panel G) and total spot number (panel H); * and ** denote significant differences compared to Sypro Ruby (P<0.05 and P<0.001; n=3).
Reprinted from Harris, LR et al. J Proteome Res. 6(4):1418-25 (2007).
1. Luo, S et al. Anal Biochem. 350(3):233–8 (2006)
2. Harris, LR et al. J Proteome Res. 6(4):1418-25 (2007)
PubMed
Shim, S et al.
Novel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly.
Mol Biol Cell. 19(6):2661-72 (2008)
Dixon, RD et al.
Palladin is an actin cross-linking protein that uses immunoglobulin-like domains to bind filamentous actin.
J Biol Chem. 283(10):6222-31 (2008)
Williams, JG et al.
An artificial processivity clamp made with streptavidin facilitates oriented attachment of polymerase-DNA complexes to surfaces.
Nucleic Acids Res.36(18):e121 (2008)
Chaurand, P et al.
Monitoring mouse prostate development by profiling and imaging mass spectrometry.
Mol Cell Proteomics. 7(2):411-23 (2008)
Harris, LR et al.
Assessing detection methods for gel-based proteomic analyses.
J Proteome Res. 6(4):1418-25 (2007)
Shibatani, T et al.
Global organization and function of mammalian cytosolic proteasome pools: Implications for PA28 and 19S regulatory complexes.
Mol Biol Cell. 17(12):4962-71 (2006)
Patterson, AD et al.
Gadd34 requirement for normal hemoglobin synthesis.
Mol Cell Biol. 26(5):1644-53 (2006)
Luo, S et al.
Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue and Fast Green.
Anal Biochem. 350(3):233–8 (2006)
Related Products
IRDye Blue Protein Stain is a convenient, safe alternative for gel staining. It is water-based and requires no hazardous solvents, unlike traditional Coomassie Blue stains, which require methanol and acetic acid for staining and destaining.
