Application Overview

Figure 1. Unstained protein standards were loaded across a 10%SDS-PAGE gel, separated by electrophoresis and stained with Coomassie Blue. The gel was imaged with the Odyssey Infrared Imaging System in the 700nm channel.
IRDye® Blue Protein Stain is a strong 700 nm fluorophore, and its fluorescence is induced upon protein binding.
Can be used for sensitive, quantitative protein detection in gels, with a linear range from ~10 ng to 20 mg1.
More sensitive than white-light imaging of Coomassie staining1-2.
IRDye Blue or Coomassie-stained SDS-PAGE gels can be imaged on any of the Odyssey Imagers with comparable sensitivity to Sypro Ruby2, but at a lower cost.
[ABOVE] Figure 2. Data courtesy of Dr. G. Sarath, University of Nebraska
[ABOVE] Figure 3. Mouse liver membrane and soluble proteins were separated on 2D gels, then stained with Sypro Ruby followed by Coomassie. Image analysis indicates differences in average SNR (panel G) and total spot number (panel H); * and ** denote significant differences compared to Sypro Ruby (P<0.05 and P<0.001; n=3).
Related Products
IRDye Blue Protein Stain is a convenient, safe alternative for gel staining. It is water-based and requires no hazardous solvents, unlike traditional Coomassie Blue stains, which require methanol and acetic acid for staining and destaining.