Odyssey Imaging System
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APPLICATIONS FOR THE ODYSSEY

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COOMASSIE® GELS


Application Overview

IRDye® Blue Protein Stain is a strong 700 nm fluorophore, and its fluorescence is induced upon protein binding.

  • Can be used for sensitive, quantitative protein detection in gels, with a linear range from ~10 ng to 20 mg1.

  • More sensitive than white-light imaging of Coomassie staining1-2.

IRDye Blue or Coomassie-stained SDS-PAGE gels can be imaged on the Odyssey Imager with comparable sensitivity to Sypro Ruby2, but at a lower cost.

1. Luo, S et al. Anal Biochem. 350(3):233–8 (2006)

2. Harris, LR et al. J Proteome Res. 6(4):1418-25 (2007)

Commassie Gels - Figure 1

Figure 1 Unstained protein standards were loaded across a 10%SDS-PAGE gel, separated by electrophoresis and stained with Coomassie® Blue.  The gel was imaged with the Odyssey Infrared Imaging System in the 700nm channel.

Coomassie® is a registered trade trademark of ICI plc.

Commassie Gels - Figure 2

[ABOVE] Figure 2. Data courtesy of Dr. G. Sarath, University of Nebraska

Commassie Gels - Figure 3

[ABOVE] Figure 3. Mouse liver membrane and soluble proteins were separated on 2D gels, then stained with Sypro Ruby followed by Coomassie. Image analysis indicates differences in average SNR (panel G) and total spot number (panel H); * and ** denote significant differences compared to Sypro Ruby (P<0.05 and P<0.001; n=3).

Reprinted from Harris, LR et al. J Proteome Res. 6(4):1418-25 (2007).

Related Products

IRDye Blue Protein Stain is a convenient, safe alternative for gel staining.  It is water-based and requires no hazardous solvents, unlike traditional Coomassie Blue stains, which require methanol and acetic acid for staining and destaining.