- Coomassie Stains & Protein Gel Documentation
- Nucleic Acid Gel Documentation
- ELISA
- EMSA/Gel Shift Assay
- Glycoprotein Detection
- In-Cell Western™ Assay
- In-Gel Western Assay
- Microwestern Array
- Northern Blot
- On-Cell Western Assay
- Protease Assay
- Protein Array
- Quantitative Western Blot
- Reporter Gene Assay
- Reverse Phase (Lysate) Array
- RNAi Analysis
- Small Animal Imaging
- Southern Blot
- Tissue Section Imaging
- Transcription Factor Assay
Email bio-eu@licor.com
APPLICATIONS for the
ODYSSEY® CLx
Applications for the Odyssey CLx Infrared Imaging System
NUCLEIC ACID GEL DOCUMENTATION
Application Overview
Sensitivity is high and background fluorescence is low when using SYTO® 60 red fluorescent nucleic acid stain to visualize DNA gels on the LI-COR Odyssey® Infrared Imagers. The detection sensitivity and lower limit of detection for SYTO 60 with any of the Odyssey Imagers are better than those of ethidium bromide detected with either a Polaroid camera or a CCD imaging system1.
If you need to image ethidium bromide, SYBR® Safe DNA stains, or other DNA stains in your nucleic acid gels in the 600 nm region, please see the Odyssey Fc information on Nucleic Acid Gel Documentation.
Figure 1.
Comparison of detection limits between ethidium bromide (Polaroid of CCD camera)
and SYTO 60 (Odyssey Infrared Imager).
| DNA Fragment (bp) | LOD (pg) | LOD (fmol) |
|---|---|---|
| 100 | 375 | 5.7 |
| 500 | 95 | 0.29 |
| 1500 | 44 | 0.044 |
Table 1. SYTO 60: Limit of Detection (LOD) with Odyssey Imaging
| DNA Fragment (bp) | LOD (pg) | LOD (fmol) |
|---|---|---|
| 100 | 3000 | 45 |
| 500 | 1500 | 4.5 |
| 1500 | 700 | 0.7 |
Table 2. Ethidium Bromide: Limit of Detection (LOD) with Polaroid Imaging
| DNA Fragment (bp) | LOD (pg) | LOD (fmol) |
|---|---|---|
| 100 | 750 | 11.4 |
| 500 | 375 | 1.14 |
| 1500 | 175 | 0.18 |
Table 3. Ethidium Bromide: Limit of Detection (LOD) with CCD Imaging
1. Syto® 60 Staining of Nucleic Acids on Gels LI-COR Biosciences (2010)
Technical Resources
Syto® 60 Staining of Nucleic Acids on Gels LI-COR Biosciences (2010)
PubMed
Davies, BS et al.
Increasing the length of progerin's isoprenyl anchor does not worsen bone disease or survival in mice with Hutchinson-Gilford progeria syndrome.
J Lipid Res. 50(1):126-34 (2009)
Poleshko, A et al.
Identification of cellular proteins that maintain retroviral epigenetic silencing: evidence for an antiviral response.
J Virol. 82(5):2313–2323 (2008)
Judge, AR et al.
Role for IkappaBalpha, but not c-Rel, in skeletal muscle atrophy.
Am J Physiol Cell Physiol. 292(1):C372-82 (2007)
Zhang, Y and L.M. Wahl.
Synergistic enhancement of cytokine-induced humanmonocyte matrix metalloproteinase-1 by C-reactive protein and oxidized LDL through differential regulation of monocyte chemotactic protein-1 and prostaglandin E2.
J Leukoc Biol. 79(1):105-113 (2006)
Davies, BSJ et al.
Dual activators of the sterol biosynthetic pathway of Saccharomyces cerevisiae: similar activation/regulatory domains but different response mechanisms.
Mol Cell Biol. 25(16):7375–7385 (2005)
