Application Overview

ELISA detection can be converted to near-infrared FLISA detection on the Odyssey System.

• Involves substitution of IRDye 800CW^® Streptavidin or IRDye 680LT Streptavidin for the horseradish peroxidase conjugate

• Eliminates the enzymatic reaction and the need to time and stop the colorimetric reaction

• Allows dry assay plates to be stored in the dark and imaged at your convenience

• Completed easily in 2.5 hours

[ABOVE] Protein A Standard was assayed, replacing the streptavidin peroxidase conjugate with IRDye^® 800CW Streptavidin. A) Odyssey image of standards assayed in duplicate. B) Graph of dilution series, displayed as concentration of Protein A vs. signal intensity. From RepliGen* Protein A ELISA Assay on the Odyssey System LI-COR Biosciences (2008) Reprinted with permission from RepliGen* Protein A ELISA Assay on the Odyssey System LI-COR Biosciences (2008).

Technical Resources

• RepliGen* Protein A ELISA Assay on the Odyssey System
  LI-COR Biosciences (2008)
  *RepliGen Corporation, Waltham, MA

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• Fluorophore-Linked Immunosorbent Assay (FLISA) Recommendations;
  Odyssey Infrared Imaging System
  LI-COR Biosciences (2006)

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• Gerthoffer, WT and Ba, M.
  Comparative Antibody Titers Using A Microtiter Plate Assay Versus Dot Blotting on Nitrocellulose
  LI-COR Biosciences (2005)

PubMed

Publishing with LI-COR data?

• Meng, H et al.
  Thrombospondin 2 potentiates notch3/jagged1 signaling.
  J Biol Chem. 284(12):7866-74 (2009)

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• Burkhead, JL et al.
  COMMD1 forms oligomeric complexes targeted to the endocytic membranes via specific interactions with phosphatidylinositol 4,5-bisphosphate.
  J Biol Chem. 284(1):696-707 (2008)

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• Mehta, AS et al.
  Increased levels of galactose-deficient anti-Gal immunoglobulin G in the sera of hepatitis C virus-infected individuals with fibrosis and cirrhosis.
  J Virol. 82(3):1259-70 (2008)

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• Walsh, ML et al.
  Fluorochrome-linked immunoassay for functional analysis of the mannose binding lectin complement pathway to the level of C3 cleavage.
  J Immunol Meth. 323(2):147-59 (2007)

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