- Coomassie Stains & Protein Gel Documentation
- Nucleic Acid Gel Documentation
- ELISA
- EMSA/Gel Shift Assay
- Glycoprotein Detection
- In-Cell Western™ Assay
- In-Gel Western Assay
- Microwestern Array
- Northern Blot
- On-Cell Western Assay
- Protease Assay
- Protein Array
- Quantitative Western Blot
- Reporter Gene Assay
- Reverse Phase (Lysate) Array
- RNAi Analysis
- Small Animal Imaging
- Southern Blot
- Tissue Section Imaging
- Transcription Factor Assay
Email bio-eu@licor.com
APPLICATIONS for the
ODYSSEY® CLx
Applications for the Odyssey CLx Infrared Imaging System
GLYCOPROTEIN DETECTION
Array Analysis
Description:
Comparative analysis for glycoproteins can be performed using the lectin based GlycoArray Kit (Qiagen, #37702).
Detection Cartoon:
The Qproteome™ GlycoArray Kit allows for four modes of detection
1. Labeled glycoprotein 
2. Labeled streptavidin for biotinylated proteins 
3. Labeled glycoprotein primary antibodies 
4. Labeled secondary antibodies. 
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Odyssey Data:
The GlycoArray contains lectins with various glycoprotein specificities (left).Each slide contains two replicated blocks of lectins. Each block has 12 squares containing nine spotted lectins in a 3 x 3 pattern. A single lectin is replicated three times in each square. The kit includes analysis software for determination of the type of glycoprotein interactions. The analysis gives structural characteristics for each glycoprotein (i.e. type of N-linked branching). The kit is not made for quantitative determination of glycoprotein content in sample, but better suited for comparative analyzes.
The GlycoArray slides were processed using the Qiagen protocol substituting IRDye labeled conjugates for visible probes. Purified lactoferrin was added to the Lactoferrin Positive slide—200 nM lactoferrin. Water was used in the negative control slide. Rabbit anti-lactoferrin primary antibody was then added, followed by IRDye® 800CW goat anti-rabbit. The slides were scanned at 21 μm on an Odyssey® Infrared Imaging System (left).
