- Coomassie Stains & Protein Gel Documentation
- Nucleic Acid Gel Documentation
- ELISA
- EMSA/Gel Shift Assay
- Glycoprotein Detection
- In-Cell Western™ Assay
- In-Gel Western Assay
- Microwestern Array
- Northern Blot
- On-Cell Western Assay
- Protease Assay
- Protein Array
- Quantitative Western Blot
- Reporter Gene Assay
- Reverse Phase (Lysate) Array
- RNAi Analysis
- Small Animal Imaging
- Southern Blot
- Tissue Section Imaging
- Transcription Factor Assay
Email bio-eu@licor.com
APPLICATIONS for the
ODYSSEY® CLx
Applications for the Odyssey CLx Infrared Imaging System
NORTHERN BLOT
Application Overview
[LEFT] Sensitivity and linearity of Cyclin G detection in mouse heart total RNA using biotin/streptavidin and the Odyssey System. Reprinted with permission from Ambroz, KLH et al. Poster presentation, PAG annual meeting (2003).
Fluorescent Detection of Northern Blots can be Performed with the Odyssey Systems
Label probe by incorporation of the desired nucleotide:
Biotin-16-dUTP*(Roche Applied Science,
catalog # 11093070910)- Detect with IRDye 800CW Streptavidin
Aminoallyl dUTP*
Label probe with reactive dye prior to hybridization
Sensitivity equivalent to chemiluminescence
Fluorescent method allows accurate quantification
No hazardous materials or radioactivity
No waiting during exposures or film processing
Sharp bands with no "blow-out”
Well suited for DNA macroarrays1
Figure 1. [ABOVE] A biotinylated GAPDH probe was hybridized to mouse heart total RNA and detected with near-infrared streptavidin and the Odyssey system. The graph shows quantification of the Northern blot results.
[LEFT] Sensitivity and linearity of Cyclin G detection in mouse heart total RNAusing biotin/streptavidin and the Odyssey System. Reprinted with permission from Ambroz, KLH et al. Poster presentation, PAG annual meeting (2003).
* LI-COR Biosciences recommends that all restrictions placed on product labels and product inserts for biotin-16-dUTP and aminoallyl dUTP be followed. Applications other than those recommended on the product inserts may require a license under certain patents owned by third parties. LI-COR Biosciences does not grant any additional license to make, use or sell these products.
1 Ambroz, KLH et al. Gene Expression Analysis Using Infrared Fluorescent Labeling and Detection Methods
Poster presentation, PAG annual meeting (2003)
Technical Resources
Northern Blot Analysis Using Biotin PCR Labeled Probes
LI-COR Biosciences (2007)Ambroz, KLH et al.
Gene Expression Analysis Using Infrared Fluorescent Labeling and Detection Methods
Poster Presentation, PAG Annual Meeting (2003)
PubMed
Owczarek-Lipska, M et al.
Bovine cardiac troponin I gene (TNNI3) as a candidate gene for bovine dilated cardiomyopathy
Archiv Tierzucht. 59(2):111-218 (2009)Liu, JM and Liu, DR.
Discovery of a mRNA mitochondrial localization element in Saccharomyces cerevisiae by nonhomologous random recombination and in vivo selection
Nucleic Acids Res. 35(20):6750-61 (2007)
