Figure 1. A probe for the UMC60 insert was labeled by PCR incorporation of a biotinylated nucleotide, using 60% Biotin-16-dUTP and 40% dTTP in the reaction. 150ng of probe used, achieving detection of ~3.15 attomoles of insert. Light blue pixels indicate saturation of signal.

Application Overview

Fluorescent Detection of Southern Blots
can be performed with the Odyssey Systems

• Label probe by incorporation of the desired nucleotide:
  Biotin-16-dUTP* (Roche Applied Science, P/N 11093070910)

  • Detect with IRDye 800CW Streptavidin

  Aminoallyl dUTP*

  • Direct direction after hybridization and washing

 

• Sensitivity equivalent to chemiluminescence

  • Fluorescent method allows accurate quantification

  • No hazardous materials or radioactivity

  • No waiting during exposures or film processing

  • Sharp bands with no "blow-out”

* LI-COR Biosciences recommends that all restrictions placed on product labels and product inserts for biotin-16-dUTP and aminoallyl dUTP be followed. Applications other than those recommended on the product inserts may require a license under certain patents owned by third parties. LI-COR Biosciences does not grant any additional license to make, use or sell these products.

Technical Resources

• Southern Blot Analysis Using Biotin-Labeled Probes
  LI-COR Biosciences

PubMed

Publishing with LI-COR data?

• Braunschweig, MH et al.
  Quantification of global DNA methylation with infrared fluorescence in liver and muscle tissues of differentially fed boars.
  Luminescence. [Epub ahead of print] (2009)

• Evans, AL et al.
  Positive selection near an inversion breakpoint on the neo-X chromosome
  of Drosophila americana.
  Genetics. 177(3):1303-19(2007)

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