Application Overview

The Odyssey Systems can image tissue sections stained with IRDye antibodies or optical probes. The sections can be labeled with standard immunohistochemical protocols or in vivo as part of an animal imaging study. Sections are imaged at 21 µM resolution for macro-level analysis. Many sections can be imaged in a single scan for screening, so that valuable microscope time is used more efficiently.

See the Microscopy page for further information on using LICOR dyes for microscope applications.

 

Key advantages of using one of the Odyssey Imagers for tissue sections:

• Quickly screens multiple sections prior to microscopic analysis.

• Permits multiplexed detection of two different targets.

• Allows quantification of staining.

• Does not require restaining after in vivo imaging experiments with IRDye optical probes.

---

---

^1. Kearn, CS. Immunofluorescent mapping of cannibinoid CB1 and dopamine D2 receptors in the mouse brain LI-COR Biosciences application note (2004)

Technical Resources

• Kearn, CS.
  Immunofluorescent mapping of cannibinoid CB1 and dopamine D2 receptors in the mouse brain
  LI-COR Biosciences application note (2004)

• Webinar: “Characterizing GPCR Function” Chris Kearn, University of Washington

PubMed

Publishing with LI-COR data?

Kobuke, K et al.
A common disease-associated missense mutation in alpha-sarcoglycan fails to cause muscular dystrophy in mice.
Hum Mol Genet. 17(9): 1201-13 (2008)

• In a study the effects of an H77C substitution in a-sarcoglycan on Limb-girdle muscular dystrophy type 2D (LGMD2D), Kobuke et al imaged tissue sections stained in vivo. Evans blue dye (EBD) is a membrane-impermeant molecule that binds to serum albumin, and is physically restricted from fibers unless the sarcolemmal membrane is damaged. They observed EBD uptake in sections taken from the quadriceps of mice after treadmill exercise.

Ghosh, A et al.
Selective inhibition of NF-ΚB activation prevents dopaminergic neuronal loss in a mouse model of Parkinson’s disease
Proc Natl Acad Sci USA. 104(47):18754-9 (2007)

• Ghosh et al. imaged mouse brain tissue sections on Odyssey following tail vein injection of a dye-labeled NBD into live mice. The data demonstrated that NBD peptide can ingress into the brain.

Kearn, CS.
Immunofluorescent mapping of cannibinoid CB1 and dopamine D2 receptors in the mouse brain
LI-COR Biosciences application note (2004)

• For higher throughput imaging of mouse brain sections, Kearn et al. employed the Odyssey Infrared Imaging System to determine basal expression patterns and co-localization of CB1 and D2 receptors in normal mouse brain to monitor changes in G protein-coupled receptor levels. This approach required less primary antibody and shorter incubation times.  It also provided an extended window for analysis relative to standard immunohistochemical staining approaches.

Roy, EJ et al.
Imaging membrane intercalating near infrared dyes to track multiple cell populations.
J Immunol Methods. 348(1-2):18-29 (2009)

Kovar, J et al.
Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models.
Anal Biochem. 384(2): 254-262 (2009)

Mondal, S et al.
Functional blocking monoclonal antibodies against IL-12p40 homodimer inhibit adoptive transfer of experimental allergic encephalomyelitis.
J Immunol. 182:5013–5023 (2009)

Hofmeister, MV et al.
Fluorescence isolation of mouse late distal convoluted tubules and connecting tubules: effects of vasopressin and vitamin D3 on Ca2+ signaling.
Am J Physiol Renal Physiol. 296(1):F194-203 (2009)

Kobuke et al.
A common disease-associated missense mutation in alpha-sarcoglycan fails to cause muscular dystrophy in mice
Hum Mol Genet. 17(9):1201-13 (2008)

Kovar, J et al.
A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models.
Anal Biochem. 367(1):1-12 (2007)

Kovar, J et al.
Purification method directly influences effectiveness of an epidermal growth factor-coupled targeting agent for noninvasive tumor detection in mice.
Anal Biochem. 361(1):47-54 (2007)

Hawes, JJ et al.
GalR1, but not GalR2 or GalR3, levels are regulated by galanin signaling in the locus coeruleus through a cyclic AMP-dependent mechanism.
J Neurochem. 93(5):1168-76 (2005)

Hawes, JJ et al.
Characterization of GalR1, GalR2, and GalR3 immunoreactivity in catecholaminergic nuclei of the mouse brain.
J Comp Neurol. 479(4):410-23 (2004)

Related Products

Odyssey® CLx Infrared Imaging System IRDye® Secondary Antibody Conjugates

IRDye® Streptavidin Conjugates IRDye® Protein Labeling Kits