Application Overview

IMPORTANT: IRDye 680LT dye products should not be used for small animal in vivo imaging.

Cell surface transporters can be used as targets for in vivo imaging by injection of agents that bind specifically to the transporter.

• Tumors are often more metabolically active than surrounding normal cells, and may have an increased rate of glucose metabolism and cell surface glucose transporter activity¹.

• This property is widely exploited in the clinical setting for PET imaging with ¹⁸FDG.

• The GLUT1 glucose transporter can be targeted with a fluorescently labeled
  IRDye^® 2-deoxyglucose (2-DG) agent for longitudinal studies of tumor progression².

 

Near-infrared dyes, such as IRDye^® fluorophores, and carefully optimized hardware are critical for high-performance optical imaging.

• Near-infrared fluorophores exploit the spectral region where light absorption and scatter properties of tissue are most advantageous³. This enhances penetration depth (access of excitation light to the fluorophore) and escape of emitted fluorescence from the animal to reach the detector.

• Laser illumination delivers very intense excitation light of the correct wavelength, generating the brightest possible signal from the fluorescent agent.

• Intrinsic autofluorescence from animal tissue can mask the signal from optical probes. In the NIR spectral region, autofluorescence is dramatically lowered^4,5.

^1. Gambhir, SS et al. Journal of Nuclear Medicine 42:1S-93S (2001)

^2. Kovar, J et al. Analytical Biochemistry 384: 254-262 (2009)

^3. Hawrysz, DJ and Sevick-Muraca, EM. Neoplasia 2(5):388–417 (2000)

^4. Frangioni, JV. Curr Opin Chem Biol. 7(5):626-34 (2003)

^5. Adams, KE, et al. J Biomed Opt. 12(2):024017 (2007)

Technical Resources

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Webinars and Video Tutorials

• Why Image in NIR?

  • “Advances in In Vivo Imaging: Near-Infrared Optical Imaging of Mice”
    Jeff Harford, LI-COR

  • “Instrumentation and Imaging Considerations”
    Eva Sevick-Muraca, Baylor College of Medicine

• Development of Imaging Agents

  • “Systematic Evaluation of Targeted IRDye^® Labeled Optical Imaging Agents”
    Joy Kovar, LI-COR

*See Small Animal Imaging application page for more related webinars.

PubMed

Publishing data from your LI-COR system?

Click a topic listed below to see all related articles

• Why Image in the Near Infrared?

  • Adams, KE et al.
    Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer.
    J Biomed Opt. 12(2):024017 (2007)

  • Kovar, J et al.
    A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models.
    Anal Biochem. 367():1-12 (2007)

  • Osterman, H and Schutz-Geschwender, A.
    Seeing beyond the visible with IRDye^® infrared dyes
    LI-COR Biosciences (2007)

  • Olive, DM.
    Near infrared technology and optical agents for molecular imaging
    LI-COR Biosciences(2006)

• Development of Imaging Agents

  • Kovar, J et al.
    Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models.
    Anal Biochem 384(2): 254-62 (2009)

  • Kovar, J et al.
    A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models.
    Anal Biochem. 367(1):1-12 (2007)

  • Kovar, J et al.
    Purification method directly influences effectiveness of an epidermal growth factor-coupled targeting agent for noninvasive tumor detection in mice.
    Anal Biochem. 361(1):47-54 (2007)

• Targeted Imaging Agents

  • Kovar, J et al.
    GLUT Family Transporters Involvement in Cellular Uptake of IRDye^® 800CW 2-Deoxyglucose.
    Poster presentation, SMI Annual Meeting (2008)

  • Kovar, J et al.
    IRDye^® 800CW 2-deoxyglucose, a near-infrared metabolic optical imaging agent.
    Poster presentation, AACR Annual Meeting (2007)

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