Use IRDye® Labeled Oligonucleotides for Safer, Faster Fluorescent Gel Shift Assays

The EMSA (electrophoretic mobility shift assay) is used to study protein:DNA complexes and interactions. Protein:DNA complexes migrate more slowly than unbound linear DNA on a non-denaturing gel, causing a “shift.”

Also called “gel shift” or “gel retardation” assays, EMSA can be used to analyze sequence-specific recognition of nucleic acids by proteins.

Traditional, radioactive EMSA protocols can be easily adapted to near-infrared fluorescence EMSA detection by using IRDye end-labeled oligonucleotides and imaging with the Odyssey® CLx or Odyssey Classic Infrared Imaging System, providing a safe and sensitive alternative.

Comparison of Detection Methods for Fluorescent Gel Shift Assay

For more information on the EMSA workflow and a sample protocol for infrared fluorescent mobility shift assays, visit our website.

Odyssey® Family of Imaging Systems: The Standard for Quantitative Western Blotting

Odyssey Family of Imaging SystemsAs a researcher, your goal is to efficiently present the most accurate data possible.

For more than 30 years, traditional chemiluminescent detection with film has provided data that have been published by scientists worldwide.

Over the past decade, LI-COR has revolutionized methods for protein detection that completely eliminate the need for film. Meet the Odyssey Imaging Systems Family!

Benefits of Digital Imaging with an Odyssey System:

Learn more about the Odyssey CLx Infrared Imaging System (supports the widest range of applications and has the largest scan surface), the Odyssey Fc Chemiluminescent and Fluorescent Imaging System (chemi and IR Westerns PLUS ethidium bromide-stained DNA gel imaging), and the Odyssey Sa Infrared Imaging System (with barcode reader and plate-reader automation options).

Not sure which system is right for your lab? Walk through our Odyssey Imager Selection Guide!

Want to see one of our LI-COR imagers in action? Let us to set up an Odyssey demonstration.