Is your capital equipment budget money tight? (when isn’t it, right?) Well, if you want to do quantitative infrared Western blotting AND plate-based assays, you should consider the Odyssey Sa Infrared Imaging System – the economical imager solution. Less expensive than the multi-functional, supports-more-than-20 applications Odyssey CLx Infrared Imaging System, the Odyssey Sa still gives you the power of infrared fluorescent technology for accurate, sensitive protein quantification.
PLUS if you need plate-based assay automation, the Odyssey Sa is perfect! With the Odyssey Sa Express Automation Software and either a 30-plate or 50-plate BioTek® BioStack™ Microplate Stacker (BioStack2WR), your lab’s throughput can increase dramatically. The barcode reader accessory (P/N 9260-61) may be added to any Odyssey Sa System for plate tracking.
If you would like a demo or more information, please let us know by completing this form.
As a summary, the Odyssey Sa Infrared Imaging System includes:
For additional information, refer to the Odyssey Sa Infrared Imaging System Brochure.
Get the Power of Infrared Technology at an Affordable Price! Happy Researching!
NewBlot Western Blot Stripping Buffers are specially formulated for use with IRDye® infrared dyes (680RD, 680LT, and 800CW only) and the Odyssey® Infrared Imaging Systems to help you save time and money on recreating samples. NewBlot Stripping Buffer allows you to reuse the same blot by stripping and reprobing up to two fluorescent antibodies simultaneously.
So, you may ask, what’s so great about NewBlot Stripping Buffer?
- Effectively removes antibodies, yet gentle enough to retain immobilized proteins
- Strips blots at room temperature in 20 minutes or less without an unpleasant odor
- Allows you to reuse the same blot up to 3X! (see the data below!)
- Offers qualitative analysis after stripping
In the example below, beta tubulin and ERK2 were run on a gel and transferred to Immobilon®-FL PVDF membrane. They were probed with primary antibodies rabbit anti-beta-tubulin and mouse anti-ERK2 and then with IRDye 680 Goat anti-Rabbit (red) and IRDye 800CW Goat anti-Mouse (green), respectively. NewBlot PVDF Stripping Buffer was used to strip the blot, which was then reprobed with the fluorescent secondary antibodies. This was repeated 2 more times. As you can see from the series of images, there is very little apparent loss of signal in either channel in the third blot as compared to the original blot.
NewBlot is available in two ‘flavors’: one for stripping nitrocellulose membranes and the other for stripping PVDF membranes.
Note: On August 25, 2014, we launched two new Western blot stripping buffers: NewBlot™ IR Stripping Buffer for infrared Western blots on either PVDF OR nitrocellulose membranes; and, WesternSure® ECL Stripping Buffer for chemiluminescent Western blot stripping and reprobing. BOTH do not require hazardous shipping charges, unlike many other Western stripping buffers.
Odyssey Infrared Imaging Systems and LI-COR® reagents have been used for neuroscience research applications in many different disciplines, including neurobiology, Alzheimer’s Disease, Parkinson’s, neural tumors, and more.
This week the Society for Neuroscience is holding its annual meeting in New Orleans, LA. LI-COR is there showcasing the Odyssey CLx and the Odyssey Fc, plus the MPX™ Multiplex Blotting System, and several new products. Stop by Booth 2713 and talk to our LI-COR representatives. Fresh chocolate chip cookies will be available Monday and Tuesday at the booth during certain times – while supplies last! (follow us on Twitter – @licorbio – to find out when to stop by and get a fresh cookie snack!)
Plus pick up your free ‘brain’ pen at the LI-COR Booth (#2713).
Enjoy the conference! We wish you continued success in your research!
Here are a few journal references related to research in the Neurosciences:
Conditional Deletion of Notch1 and Notch2 Genes in Excitatory Neurons of Postnatal Forebrain Does Not Cause Neurodegeneration or Reduction of Notch mRNAs and Proteins
Jin Zheng, Hirotaka Watanabe, Mary Wines-Samuelson, Huailong Zhao, Thomas Gridley, Raphael Kopan, and Jie Shen
J. Biol. Chem., Jun 2012; 287: 20356 – 20368.
Enhancement of Rostral Ventrolateral Medulla Neuronal Nitric-Oxide Synthase–Nitric-Oxide Signaling Mediates the Central Cannabinoid Receptor 1-Evoked Pressor Response in Conscious Rats
Badr Mostafa Ibrahim and Abdel A. Abdel-Rahman
J. Pharmacol. Exp. Ther., Jun 2012; 341: 579 – 586.
Specific Serine-Proline Phosphorylation and Glycogen Synthase Kinase 3β-directed Subcellular Targeting of Stathmin 3/Sclip in Neurons
Sara Devaux, Fabienne E. Poulain, Véronique Devignot, Sylvie Lachkar, Theano Irinopoulou, and André Sobel
J. Biol. Chem., Jun 2012; 287: 22341 – 22353.
For more journal references citing the use of the Odyssey Imagers and LI-COR reagents, see our most recent Publications List.
IRDye 800CW YC-27 (available through LI-COR Custom Services) is a near-infrared dye-labeled imaging agent specifically designed to target prostate specific membrane antigen (PSMA), also known as folate hydrolase I or glutamate carboxypeptidase II.
This small molecule can be used as an optical imaging agent for in vitro (such as In-Cell Western™ Assays), in vivo, whole organ, and tissue section analysis, allowing the same probe to be used in all steps of the biomarker discovery process.
Figure 1. Example of tumor imaging with IRDye 800CW YC-27. Nude mouse bearing 22Rv1 xenograft tumor on the right hip (white arrow) received IRDye 800CW YC-27 (0.5 nmole) 24 hours prior to imaging on the Pearl® Small Animal Imaging System. Orange arrows point to residual kidney clearance of optical imaging agent.
PSMA is a type II glycoprotein that is over-expressed in prostate cancer including metastatic disease. PSMA is also expressed on the tumor vascular endothelium of virtually all solid carcinomas and sarcomas but not on normal vascular endothelium. This expression suggests a potential mechanism for specific targeting of tumor-associated neovasculature. IRDye 800CW YC-27 (urea-based small molecule; MW 1743) has been characterized for in vitro and in vivo use with a number of tumor cell lines which include LNCaP, 22Rv1, PC3M-LN4 (prostate carcinomas), PC3-PIP (PC3 cells transfected with PSMA) and PC3-flu (PSMA-). These characteristics make it ideal for preclinical evaluation of PSMA-expressing tissue such as prostate tumors.
For information on BrightSite™ Small Animal Imaging Agents labeled with IRDye near-infrared fluorescent dyes, visit our LI-COR BIO website.
Would you like to label your own compounds with with NIR fluorescent dyes? Try one of our IRDye Protein Labeling Kits.