Reprobe Fluorescent Westerns with NewBlot™ Western Blot Stripping Buffers

NewBlot Stripping Buffer IconNewBlot Western Blot Stripping Buffers are specially formulated for use with IRDye® infrared dyes (680RD, 680LT, and 800CW only) and the Odyssey® Infrared Imaging Systems to help you save time and money on recreating samples. NewBlot Stripping Buffer allows you to reuse the same blot by stripping and reprobing up to two fluorescent antibodies simultaneously.

So, you may ask, what’s so great about NewBlot Stripping Buffer?

  • Effectively removes antibodies, yet gentle enough to retain immobilized proteins
  • Strips blots at room temperature in 20 minutes or less without an unpleasant odor
  • Allows you to reuse the same blot up to 3X! (see the data below!)
  • Offers qualitative analysis after stripping

In the example below, beta tubulin and ERK2 were run on a gel and transferred to Immobilon®-FL PVDF membrane. They were probed with primary antibodies rabbit anti-beta-tubulin and mouse anti-ERK2 and then with IRDye 680 Goat anti-Rabbit (red) and IRDye 800CW Goat anti-Mouse (green), respectively. NewBlot PVDF Stripping Buffer was used to strip the blot, which was then reprobed with the fluorescent secondary antibodies. This was repeated 2 more times. As you can see from the series of images, there is very little apparent loss of signal in either channel in the third blot as compared to the original blot.
Example of using NewBlot Stripping Buffer on PVDF Membranes

NewBlot is available in two ‘flavors’: one for stripping nitrocellulose membranes and the other for stripping PVDF membranes.

Get more power out of your blot with NewBlot Western Blot Stripping Buffers!

Note: On August 25, 2014, we launched two new Western blot stripping buffers: NewBlot™ IR Stripping Buffer for infrared Western blots on either PVDF OR nitrocellulose membranes; and, WesternSure® ECL Stripping Buffer for chemiluminescent Western blot stripping and reprobing. BOTH do not require hazardous shipping charges, unlike many other Western stripping buffers.

Odyssey® References for Neuroscience Research Applications

Otis Spunkmeyer Chocolate Chip Cookie Odyssey Infrared Imaging Systems and LI-COR® reagents have been used for neuroscience research applications in many different disciplines, including neurobiology, Alzheimer’s Disease, Parkinson’s, neural tumors, and more.

This week the Society for Neuroscience is holding its annual meeting in New Orleans, LA. LI-COR is there showcasing the Odyssey CLx and the Odyssey Fc, plus the MPX™ Multiplex Blotting System, and several new products. Stop by Booth 2713 and talk to our LI-COR representatives. Fresh chocolate chip cookies will be available Monday and Tuesday at the booth during certain times – while supplies last! (follow us on Twitter – @licorbio – to find out when to stop by and get a fresh cookie snack!)
LI-COR Brain Pen

Plus pick up your free ‘brain’ pen at the LI-COR Booth (#2713).

Enjoy the conference! We wish you continued success in your research!

Here are a few journal references related to research in the Neurosciences:

Conditional Deletion of Notch1 and Notch2 Genes in Excitatory Neurons of Postnatal Forebrain Does Not Cause Neurodegeneration or Reduction of Notch mRNAs and Proteins
Jin Zheng, Hirotaka Watanabe, Mary Wines-Samuelson, Huailong Zhao, Thomas Gridley, Raphael Kopan, and Jie Shen
J. Biol. Chem., Jun 2012; 287: 20356 – 20368.

Enhancement of Rostral Ventrolateral Medulla Neuronal Nitric-Oxide Synthase–Nitric-Oxide Signaling Mediates the Central Cannabinoid Receptor 1-Evoked Pressor Response in Conscious Rats
Badr Mostafa Ibrahim and Abdel A. Abdel-Rahman
J. Pharmacol. Exp. Ther., Jun 2012; 341: 579 – 586.

Specific Serine-Proline Phosphorylation and Glycogen Synthase Kinase 3β-directed Subcellular Targeting of Stathmin 3/Sclip in Neurons
Sara Devaux, Fabienne E. Poulain, Véronique Devignot, Sylvie Lachkar, Theano Irinopoulou, and André Sobel
J. Biol. Chem., Jun 2012; 287: 22341 – 22353.

For more journal references citing the use of the Odyssey Imagers and LI-COR reagents, see our most recent Publications Lists.