Just like all of the LI-COR IRDye secondary antibodies, these are highly cross-adsorbed secondary antibody conjugates suitable for a variety of applications (see the table below).
IRDye 800CW secondary antibodies are the antibodies of choice for a wide variety of applications in the 800 nm channel (see the list below). IRDye 800CW secondary antibodies can be used for 2-color detection when multiplexed with IRDye 680RD or IRDye 680LT secondary antibodies.
IRDye 680RD secondary antibodies are the antibodies of choice for In-Cell Western Assay and Western blot applications in the 700 nm channel. These antibodies can be used for 2-color detection when multiplexed with IRDye 800CW secondary antibodies. These antibodies are our most universal use 700 nm channel antibodies.
IRDye 680LT secondary antibodies have been proven the brightest signal for Western blot detection in the 700 nm channel and are comparable to Alexa Fluor 680 secondary antibodies. They are an excellent choice for low abundance targets and can be used for 2-color detection when multiplexed with IRDye 800CW secondary antibodies.
|Application||IRDye 800CW Secondaries||IRDye 680RD Secondaries||IRDye 680LT Secondaries|
|In-Cell Western™ Assay||√||√||Not Recommended|
|On-Cell Western Assay||√||√||Not Recommended|
|2D Gel Detection||√||√||√|
|Tissue Section Imaging||√||√||√|
|Small Animal Imaging||√||√||Not Recommended|
|Virus Titration Assay||√||Not Known||Not Known|
|FRET-based Assay||√||Not Known||Not Known|