What is the future of medical film?
Nearly a year ago we told you why film’s future availability and affordability are in jeopardy. Today, we are still seeing a decreased demand and reduced production volume of film. But there are additional concerns. The environment is suffering because of the hazardous chemical and medical waste produced from using film.
Here are some realities facing Western blotters who use medical film:
- The federal Resource Conservation and Recovery Act (RCRA) sets regulations for hazardous waste handling and storage.
- The RCRA has strict laws with authority from the EPA enforcing toxic chemical cleanup.
- Developer solutions must be neutralized and flushed with large quantities of water to the sewer system.
- Film sheets should be collected for silver recycling because silver is too toxic to go in landfills.
What are the implications?
As environmental concerns rise and the supply of film is threatened, the sustainability and future of film production are at risk. As a responsible research scientist, you are aware there are environmental considerations and financial incentives for ceasing film use and switching to digital imaging. Read about one researcher who has come to that realization.
What can you do?
Consider an environmentally-friendly Western blot imaging alternative, and:
- Eliminate your use of medical film
- Decrease your environmental impact
- Implement a more sustainable Western blotting technique
Go to bed at night without worrying if you can afford your next box of film or if you are complying with environmental hazardous waste disposal regulations. Go digital.
Does your lab have both researchers that use PVDF membranes and researchers that use nitrocellulose for infrared Western blots? Are you still spending your valuable time making homemade stripping buffer?
NewBlot™ IR Stripping Buffer to the rescue! NewBlot IR is the latest member of the LI-COR NewBlot Stripping Buffer family.
All NewBlot buffers can be used for stripping and reprobing infrared fluorescent Western blots, and there is a formulation that works for whichever membrane type (or types) you use.
What makes NewBlot IR unique?
- It strips both membrane types so you won’t need to buy separate stripping buffers for nitrocellulose and PVDF.
- It is the most affordable, saving you money to spend on other items for your lab.
This robust reagent removes primary and secondary antibodies while maintaining target antigen integrity for efficient reprobing and does not require hazardous shipping, unlike many other stripping buffers.
Note: NewBlot IR Stripping Buffer is not recommended for loading amounts over 30 µg.
Below are data showing that you can indeed strip and reprobe three times and still detect your proteins of interest.
Figure 1. Strip and reprobe nitrocellulose or PVDF membranes effectively with NewBlot IR Stripping Buffer. EGFR and phospho-ERK levels were compared in EGF-stimulated (+) and non-stimulated (-) A431 lysate (1 µg total protein). The membrane was probed with mouse anti-EGFR, mouse anti-pERK1/2, and β-tubulin rabbit polyclonal (LI-COR P/N 926-42211), then detected with IRDye 800CW Goat anti-Mouse (LI-COR P/N 926-32210) and IRDye 680RD Goat anti-Rabbit (LI-COR P/N 926-68071). The blot was scanned with an Odyssey® CLx Imaging System (Original blot). The blot was then stripped with NewBlot IR Stripping Buffer (LI-COR P/N 928-40028), and scanned again (Strip #1). The blot was detected with the same primary and secondary antibodies and scanned again (Reprobe #1). The process was repeated 2 more times (Strip and Reprobe #2-3). Image display settings for all stripped and reprobed images are identical to the original image.
To make sure you are successful when stripping and reprobing, here are some factors that can affect stripping efficiency:
- Amount of time the blot is in the stripping buffer
- Sample type and preparation
- Blot handling conditions
- Buffer concentration and temperature used for stripping
Save your valuable time and money! Order NewBlot IR Stripping Buffer today for your infrared fluorescent Western blot stripping and reprobing needs.