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Monthly Archives: April 2012
Now Use the Same Primary Antibody for Immunoprecipitation AND Western Blotting!
LI-COR® has just rolled out a new way that the recently-released Quick Western Kit – IRDye® 680RD (see Would you like to save at least 90 minutes the next time you do a Western blot?) can be used in your … Continue reading
Posted in IRDye Reagents, Odyssey Imaging Systems, Proteomics Applications, Western Blotting
Tagged fast western blotting, immunoprecipitation, IP, irdye infrared dyes, li-cor western blots, li-cor western blotting, licor odyssey imagers, near-infrared fluorescent reagents, odyssey applications, quick western kit, western blots, Western blotting kits
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How Can You Use Quantitative Cell Signaling Analysis in Your Research? How About for IC50 Determinations?
In a previous post, I talked about how In-Cell Western™ assays could be used when studying apoptosis. So, you may be asking yourself, for what other applications can quantitative cell signaling analysis be used? GREAT QUESTION!! Well, in-Cell ELISAs (as … Continue reading
Posted in Cancer Research, Neuroscience, Odyssey Imaging Systems, Proteomics Applications
Tagged cell based assays, determining IC50s anc EC50s, ec50 determinations, IC50 determination, in-cell ELISA, li-cor odyssey, licor odyssey imagers, near-infrared fluorescent reagents, protein phosphorylation, signal transduction
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Seeding Cells in Microplates for In-Cell Western™ Assays – Hints & Tips
One of the first steps in an In-Cell Western Assay experiment is to seed cells into the wells of a tissue culture microplate. Cell density is more important for some cell lines than others. In particular, cells that depend more … Continue reading
Quantitative Cell Signaling Analysis – What’s all the Buzz About?
What’s all this BUZZZZ you are hearing about being able to quantitate cell signaling in plate-based assays? If you are at AACR in Chicago this week, stop by Booth 3800 (LI-COR® Biosciences) and we can tell you all about the … Continue reading
Posted in Cancer Research, Odyssey Imaging Systems, Proteomics Applications
Tagged cell-based ELISA, chemiluminescent detection, cytoblot, in-cell ELISA, li-cor odyssey, near-infrared fluorescent reagents, odyssey applications, protein phosphorylation, protein quantitation, quantification, signal transduction
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