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Monthly Archives: May 2012
Suspension Cells for Quantitative Cell Signaling Analysis using In-Cell Western™ Assays
So I’ve talked about how to ensure that suspension cells attach to plates, when to know you have a monolayer, and why round bottom plates are the best when doing In-Cell Western Assays with suspension cells in the 23-May-12 blog … Continue reading
Posted in Odyssey Imaging Systems, Proteomics Applications
Tagged apoptosis, cell based assays, immunofluorescent assays, in-cell ELISA, licor odyssey imagers, near-infrared fluorescent reagents, odyssey applications, signal transduction, using cells in suspension, using non-adherent cells in plate-based assays
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Washing Gently and Round Bottom Plates are Essential for Successful In-Cell ELISAs Using Non-Adherent Cells
I mentioned in my post on 23-May, that the next few entries would be on more hints and tips of how to use non-adherent cells for In-Cell Western Assays – so here goes! During my washing steps, cells are coming … Continue reading
Posted in Odyssey Imaging Systems, Proteomics Applications
Tagged cell based assays, ICE assays, ICW assays, in-cell ELISA, near-infrared fluorescent reagents, odyssey applications, plate-based assays, signal transduction, using cells in suspension, using non-adherent cells in plate-based assays, using nonadherent cells for icw, using suspension cells
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Receptor-based Binding and Competition Assays with Infrared Fluorescence
What is an On-Cell Western Assay? It’s a cell-based assay that enables quantitative monitoring of cell surface protein expression. The On-Cell Western assay offers the ability to: Detect and quantify target proteins localized to the cell surface Quantify ligand binding … Continue reading
Posted in Neuroscience, Odyssey Imaging Systems, Proteomics Applications
Tagged cannabinoid receptors, cell based assays, cell surface biotinylation assays, cell surface protein expression, in-cell ELISA, internalization assays, near-infrared fluorescent reagents, odyssey applications, plate-based assays, protein trafficking
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Image Studio Software Compatible with Mac® Systems Now Available from LI-COR!
Image Studio is an extremely simple and easy-to-use imaging software. It is compatible with the Odyssey® CLx, Odyssey Classic, and Odyssey Fc Imaging Systems. Easy to use – training for new users is fast and simple Intuitive, application-driven ribbon interface … Continue reading
