Monthly Archives: June 2012

In-Cell Western™ Webinar – Applications Review

Posted on June 28, 2012 by licor-admin

For those of you that like to watch videos and listen to information, here is a great webinar on In-Cell Western Assays. In this webinar, the basics of ICW assays are covered and these applications: Cell proliferation assays RNAi knockdowns … Continue reading →

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Normalize Protein Concentrations with Nucleic Acid Stains in In-Cell Western™ Assays

Posted on June 25, 2012 by licor-admin

When performing the In-Cell Western assay, one fluorescence channel is often used for normalization. This allows quantification data for the protein of interest to be corrected for well-to-well variation in cell number, thereby increasing the overall accuracy of the assay. … Continue reading →

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In-Cell Western™ Assay Application: Response of COS-7 Cells to Hydroxyurea

Posted on June 15, 2012 by licor-admin

Application: Detecting phospho-p53 in COS cells in response to Hydroxyurea The response of COS-7 cells to increasing doses of hydroxyurea was measured by a specific antibody (Anti-phospho-p53 from Cell Signaling Technology, P/N 9286) that detects phosphorylated-p53 (Ser16). Total ERK1 was … Continue reading →

Posted in Cancer Research, IRDye Reagents, Odyssey Imaging Systems, Proteomics Applications | Tagged , , , , , , , | Leave a comment

In-Cell Western™ Assay Quality Assessment using Z’-Factor

Posted on June 7, 2012 by licor-admin

If you have been following my posts for the past two months or so, you know we have been looking a great deal at In-Cell Western Assays (also called In-Cell ELISAs or plate-based immunofluorescent assays). On April 3, I explained … Continue reading →

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