Western blotting is the most widely used method for the detection and characterization of proteins. Although the basic elements of Western blotting remain unchanged, journal standards for publishing Western blots (e.g., JBC’s Instructions for Authors) have become more rigorous in recent years.
Are you interested in quantifying your proteins on your Western blot but are not sure how to manage Western blot variability and increase the accuracy of your results?
The key is to maximize Western blot accuracy and precision. This makes relative comparisons meaningful. How can you accomplish this? By reducing variability whenever possible with good experimental design. You can also correct for variability by using the appropriate internal loading controls for your Western blot normalization.
LI-COR developed a series of protocols to help improve the quality of quantitative Western blots. Whether you are a beginner or a seasoned user, we can help you collect rock-solid data that will meet even the toughest publication standards.
The protocols cover key factors for success, data analysis and interpretation, and include links to additional educational resources for quantitative Western blotting.
- Determining the Linear Range for QWB Detection
- Housekeeping Protein Validation Protocol
- REVERT™ Total Protein Stain Normalization Protocol
- Housekeeping Protein Normalization Protocol
- Pan/Phospho Analysis For Western Blot Normalization
Do you need help determining the linear range of your target protein and internal loading control, or validating your housekeeping protein, using REVERT total protein stain for normalization or using total and post-translationally modified proteins for normalization? If so, our tools, products, and services can help you get published.
These protocols are also available in an online format at protocols.io
Do you have a question about your Western blot normalization strategy or how publication requirements for Western blots have been changing? Contact us and let our experts help!