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Category Archives: Cancer Research
New! Optical Probe for Tumor Imaging – IRDye® 800CW YC-27
IRDye 800CW YC-27 (P/N 926-27000) is a near-infrared dye-labeled imaging agent specifically designed to target prostate specific membrane antigen (PSMA), also known as folate hydrolase I or glutamate carboxypeptidase II. This small molecule can be used as an optical imaging … Continue reading
Posted in Cancer Research, IRDye Reagents, In Vivo Imaging System and Applications, Reagents
Tagged cancer imaging, dna-protein interactions, in vitro imaging, near-infrared dyes, near-infrared fluorescent dyes, near-infrared fluorescent reagents, pearl impulse small animal imaging system, preclinical evaluation of tumors, preclinical research, prostate cancer, psma expressing tissues, targeting agent for prostate cancer, tissue section imaging, tumor imaging
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In-Cell Western™ Assay Application: Response of COS-7 Cells to Hydroxyurea
Application: Detecting phospho-p53 in COS cells in response to Hydroxyurea The response of COS-7 cells to increasing doses of hydroxyurea was measured by a specific antibody (Anti-phospho-p53 from Cell Signaling Technology, P/N 9286) that detects phosphorylated-p53 (Ser16). Total ERK1 was … Continue reading
Posted in Cancer Research, IRDye Reagents, Odyssey Imaging Systems, Proteomics Applications
Tagged cell based assays, immunofluorescent assays, in-cell ELISA, licor odyssey imagers, near-infrared fluorescent reagents, odyssey applications, protein phosphorylation, signal transduction
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How Can You Use Quantitative Cell Signaling Analysis in Your Research? How About for IC50 Determinations?
In a previous post, I talked about how In-Cell Western™ assays could be used when studying apoptosis. So, you may be asking yourself, for what other applications can quantitative cell signaling analysis be used? GREAT QUESTION!! Well, in-Cell ELISAs (as … Continue reading
Posted in Cancer Research, Neuroscience, Odyssey Imaging Systems, Proteomics Applications
Tagged cell based assays, determining IC50s anc EC50s, ec50 determinations, IC50 determination, in-cell ELISA, li-cor odyssey, licor odyssey imagers, near-infrared fluorescent reagents, protein phosphorylation, signal transduction
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Seeding Cells in Microplates for In-Cell Western™ Assays – Hints & Tips
One of the first steps in an In-Cell Western Assay experiment is to seed cells into the wells of a tissue culture microplate. Cell density is more important for some cell lines than others. In particular, cells that depend more … Continue reading
Quantitative Cell Signaling Analysis – What’s all the Buzz About?
What’s all this BUZZZZ you are hearing about being able to quantitate cell signaling in plate-based assays? If you are at AACR in Chicago this week, stop by Booth 3800 (LI-COR® Biosciences) and we can tell you all about the … Continue reading
Posted in Cancer Research, Odyssey Imaging Systems, Proteomics Applications
Tagged cell-based ELISA, chemiluminescent detection, cytoblot, in-cell ELISA, li-cor odyssey, near-infrared fluorescent reagents, odyssey applications, protein phosphorylation, protein quantitation, quantification, signal transduction
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Studying bone growth, changes, tumors? Try IRDye® BoneTag™ Optical Probes.
IRDye BoneTag optical imaging agents are tetracycline derivatives that incorporate into mineralizing bone. Structural imaging of bone can be used to more precisely localize an area of disease. A second disease-specific targeting agent with a spectrally-distinct fluorescent label can be … Continue reading
Advantages of using PSVue® 794 for Imaging Apoptosis
PSVue® 794 is a near-infrared fluorescent probe for detection of apoptotic and necrotic cells, bacteria, and other anionic membranes. The compound exhibits fluorescence excitation maximum at 794nm and emission maximum at 810 nm and through its zinc(II)-dipicolylamine (Zn-DPA) moiety, it … Continue reading
