Article Category: Software & Accessories

Odyssey CLx User, Dr. Pierre-Jacques Hamard Studies AML and Epigenetic-based Therapies

Dr. Pierre-Jacques Hamard is researching ways to put the brakes on acute myeloid leukemia (AML) and other hematopoietic diseases. As an Associate Scientist for the Nimer Lab in the Sylvester Comprehensive Cancer Center, at the University of Miami, he and his colleagues are researching the role of epigenetic factors in normal and malignant hematopoiesis.

In particular, Dr. Hamard’s research involves determining the effect protein arginine methyltransferase-5 (PRMT5) may have on DNA repair in hematopoietic cells. This line of research recently made it to the cover of the high-profile publication Cell Reports.1

Another facet of Dr. Hamard’s research has a more therapeutic slant, as he and his colleagues test the efficacy of various epigenetic-based therapies.

One such therapy they are exploring is inhibiting the expression of the protein CARM1. Dr. Hamard and his colleagues have shown that CARM1 “is important for leukemia cells but not for normal cells.”2

Their approach is “to show that inhibiting these proteins could be a viable therapeutic approach for some of the diseases that we work on such as AML.”

An Advocate of Near-Infrared Imaging

The Nimer Lab isn’t Dr. Hamard’s first experience with an Odyssey® Infrared Imager. He had previously used the imaging system during his time at the Icahn School of Medicine at Mount Sinai in New York City. In fact, when he came to Miami Dr. Hamard explained the benefits of an Odyssey Imaging System to his principal investigator, Dr. Nimer. In Dr. Hamard’s opinion, a key feature of the imager is its capability, along with Image Studio™ software, to provide quantitative Western blot data.

“I like the quantification feature of the software. It’s one of the arguments that I usually use when I want to convince people that it’s the way to go with Western blots. For me, that’s the best thing about the instrument.”

Eventually, Dr. Hamard succeeded in acquiring an Odyssey CLx. Not only does he now do approximately 95% of his own Western blots using the Odyssey CLx, Dr. Hamard says others in his lab have come to appreciate the imager, as well.

Reliable Multiplex Western Blotting

Another feature of the Odyssey CLx that Dr. Hamard has come to rely on is multiplexing Western blots. In his research, the capability to multiplex his blots is crucial. A multiplex Western blot allows Dr. Hamard to detect and assess modifications PRMT5 or CARM1 have made to a histone in relation to that histone’s total protein, regardless of modification.

“We do a lot of multiplex Western blots where we use one color for the modification, be it methylation/acetylation and so on, and another color for the total protein.”

Multiplexing his Western blots has saved Dr. Hamard time by allowing him to test multiple conditions at once and ensuring he’s using the proper reagents for his research. It also allows him to better characterize the suitability of antibodies for the experiments he is performing.

We thank Dr. Hamard for his contributions to science and are proud to call him an Odyssey CLx Expert.

Publications resulting from work on the Odyssey CLx

  1. PRMT5 Regulates DNA Repair by Controlling the Alternative Splicing of Histone-Modifying Enzymes. Hamard PJ, Santiago GE, Liu F, Karl DL, Martinez C, Man N, Mookhtiar AK, Duffort S, Greenblatt S, Verdun RE, Nimer SD. Cell Reports. 2018 Sep 4;24(10):2643-2657. doi: 10.1016/j.celrep.2018.08.002. PMID:30184499
  2. CARM1 Is Essential for Myeloid Leukemogenesis but Dispensable for Normal Hematopoiesis. Greenblatt SM, Man N, Hamard PJ, Asai T, Karl D, Martinez C, Bilbao D, Stathias V, McGrew-Jermacowicz A, Duffort S, Tadi M, Blumenthal E, Newman S, Vu L, Xu Y, Liu F, Schurer SC, McCabe MT, Kruger RG, Xu M, Yang FC, Tenen D, Watts J, Vega F, Nimer SD. Cancer Cell. 2018 Jun 11;33(6):1111-1127.e5. doi: 10.1016/j.ccell.2018.05.007. PMID: 29894694

Export Data and Print Lab Reports from LI-COR® Image Studio™ Lite Software

WOO HOO! You are done analyzing your Western blot data with Image Studio Lite. SUPER JOB!!

Now, I bet you want to export your data and create a lab report to give to your boss, right? Image Studio Lite offers the ability to export data for use in spreadsheet programs such as Microsoft Excel – and this video tutorial will walk you just how to do this.

In this last video tutorial, you will learn how to print a lab report from Image Studio Lite software. You can create standardized or custom lab reports (add your institution logo, lab name, etc) that can be shared with colleagues — and your boss! — or used to create daily research logs.

If at any time you need a refresher on how to use this free Western blot analysis software, you can view all of the tutorial videos and access all the support documents on our Image Studio Support page.

Image Studio Lite – A Dream Come True, Without the Unicorn

Set the Background and Adjust the Image Appearance in Image Studio™ Lite

Glad you asked! 🙂 So, you’ve started your Western blot analysis in Image Studio Lite. Great! (For a refresher, see Using Image Studio Lite, the Free Western Blot Analysis Software – Image Display and Analysis.) But what about setting the right background subtraction method? Hum. . . . .well, this short video tutorial will walk you through exactly how to do this.

Background subtraction method selected – check!

Okay, next you might want to adjust your Western blot image appearance. This can be done by using the Brightness/Contrast slider located on the right hand side of the screen. This quick video tutorial also shows you how you can do more advanced adjustments by using image Lookup histograms or Lookup tables.

Wow! Your Western blot image analysis is looking pretty darn good! Great job!

Have you told your friends about Image Studio Lite? Be sure to spread the word about this easy-to-use (and FREE!) Western blot analysis software. Just tell them to go download their free copy right away. And, now that you have reviewed the tutorials so far – you can help them really get going fast!

Next up – exporting data and printing lab reports.

To see all of the tutorial videos and support documents, visit the Image Studio Support page.

Image Studio Lite – A Dream Come True, Without the Unicorn

Using Image Studio™ Lite Software: Image Display and Analysis

Okay, if you followed the last post on creating a work area and importing images into Image Studio Lite, you are now ready to learn how to easily alter the image display. You can adjust the way the image is displayed in Image Studio Lite by using the “Choose Display Assistant” or chooser.

Once you have imported your Western blot image and have adjusted it to be just how you like it, you can now add shapes and begin analysis in Image Studio Lite. Luke Miller (of ImageJ tutorial fame) also wrote some really informative, helpful instructions on how to use Image Studio Lite.

Wow! That was pretty easy, wasn’t it? If you haven’t yet, GO!, download a copy of this free Western Blot analysis software from LI-COR now! To see all of the tutorial videos and support documents, visit the Image Studio Support page.

(And, again, bonus brownie points for telling your friends about Image Studio Lite! :-))

Image Studio Lite – A Dream Come True, Without the Unicorn

Create a Work Area and Import an Image into this Free Western Blot Analysis Software

In this series of posts, you will discover how easy it is to use Image Studio™ Lite. This free Western blot analysis software from LI-COR® allows you to easily create your own work area and then import images from numerous sources. So, if you have an old film scan, or an image from another chemiluminescent Western blot imaging system, try using this free Western blot analysis software. In subsequent posts, we will talk about the other functionalities of Image Studio Lite.

The first video in this post will show you how to open Image Studio Lite software and create a work area. (If you need to find out the installation requirements for Image Studio Lite, see the 17-Jul-13 Post, Computer Requirements to Get Started with Free LI-COR® Image Studio Lite Software).

This second video walks through the steps needed to import an image into Image Studio Lite. Image Studio Lite analyzes images in the tif, png, or jpeg format as well as images acquired with past versions of the Odyssey® or Pearl® imaging systems software.

Download FREE Image Studio Lite Western Blot Analysis Software from LI-COR and get started right away! (Bonus points for telling your friends about it too! :-))

Image Studio Lite – A Dream Come True without the Unicorn!

Computer Requirements to Get Started with Free Image Studio Lite 3.1

Image Studio Lite - Free Western Blot Analysis Software from LI-CORImage Studio™ Lite 3.1 is Western blot analysis software that is compatible with most image files and systems and is available for FREE download from the LI-COR website. Here are 3 short videos that will get you up and running quickly with Image Studio Lite, whether you have a Mac®-based system or a Windows®-based system.

For additional video tutorials and support documents to optimize your use of Image Studio Lite, visit our Image Studio Lite Support page.

Haven’t downloaded Image Studio Lite 3.1 yet? Do it now – and tell your friends about it, too!

Multiplex Western Blotting System Turbo-Charges Western Blot Results Output

Example of Multiplex Western Blotting using the MPX Blotting SystemMultiplex Western blotting is a powerful tool that allows you to get more out of your Western blots. Multiplex detection becomes possible when you utilize the MPX™ (Multiplex) Blotting System and LI-COR IRDye® near-infrared fluorescent dye-labeled secondary antibodies.

Multiplex Westerns can be imaged on any of the Odyssey® Imagers and provide results for a possible maximum of 48 targets on a single membrane — 24 per channel with two-color detection — and the option for quantitative analysis, saving you time and reagents! The MPX Blotting System can be used if you need to optimize:

  • Primary antibodies – to determine the primary antibody that has the right specificity and the right dilution for use
  • Antibody incubation times
  • Blocking conditions – which blocking buffer will give you the optimum results
  • Secondary antibodies – what dilutions is best to use without getting a lot of non-specific binding?
  • Or just about anything else you need to optimize!

Watch this 4 minute video on how easy it is to get the most out of multiplexing with the MPX Blotting System. You can also download the handy MPX Blotter User Guide.

Easy-to-Use Image Studio v3.1 Software Now Available for all Odyssey® and Pearl® Imaging Systems

Image Studio™ Software
from LI-COR® is available for Mac® and Windows® computers. This intuitive, easy-to-use software allows researchers to:

  • Create individual work spaces
  • Import tif, jpg, or png images from Odyssey, Pearl®, or other imaging systems
  • Image and export data
  • Customize lab notebooks

Upgrade your current Odyssey or Pearl Imager software and all the benefits of this new, improved easy-to-use Image Studio Software.

If you would like to get an idea of how easy Image Studio Software is to use, try our free version – Image Studio Lite. Image Studio Lite allows you to analyze Western blot images but does not operate your LI-COR instrument to acquire images. Try it FREE – download now! Then, tell your friends – they are sure to want a copy, too!

FREE Western Blot Analysis Software from LI-COR® – Image Studio Lite!

FREE Image Studio Lite Software Download - Western Blot Analysis
Image Studio™ Lite is Western blot analysis software that is compatible with most image files and systems and is available as a FREE download from the LI-COR website.

Just fill in the form and you will receive an email with a link to your Image Studio Lite download (Mac® and Windows® versions available). Get up to speed quickly by watching the short tutorial videos and learn about all of the features and functions of Image Studio Lite.

With this free Western blot analysis software, you can:

  • Import images from numerous sources
  • Adjust brightness/contrast
  • Define bands using shapes tools
  • Obtain relative fluorescent units for shapes
  • Export images and data
  • Create lab notebooks

What can you do with Image Studio Lite? Find out in this short introductory video!

Image Studio Software Compatible with Mac® Systems Now Available from LI-COR!

Image Studio Software for Mac and PC

Image Studio is an extremely simple and easy-to-use imaging software. It is compatible with the Odyssey® CLx, Odyssey Classic, and Odyssey Fc Imaging Systems.

  • Easy to use – training for new users is fast and simple
  • Intuitive, application-driven ribbon interface
  • Supports nine different types of analyses, including Western blots, DNA gel documentation, and small animal imaging

NOW! Compatible with Mac and PC computers!

For more information on Image Studio Software for Mac and PC, visit our website.