Analysis of glycoproteins requires sensitive and quantitative applications. LI-COR offers a single, optimized solution using the Odyssey^® Systems and IRDye^® labeled conjugates. This solution provides sensitive and quantitative results using two-color near-infrared detection at 700 nm and 800 nm wavelengths. Operating at this wavelength produces lower background from biological materials, buffer components, and standard membranes used in Western blotting and lectin binding applications and, thus, superior data.

Outlined below are a variety of applications for several one-color, visible glycoprotein applications that have been adapted to near-infrared fluorescence detection on an Odyssey Imaging System:

• Total Glycoprotein and Total Protein

o Using Glycoprotein and Coomassie Stains

• Indirect Lectin Detection

o Biotinylated Lectins using IRDye Streptavidin

• Direct Lectin Detection

o Using IRDye Protein Labeling Kits

• Glycoprotein Antibody Detection

o Using IRDye Secondary Antibodies

• Enzymatic Detection

o Using IRDye Streptavidin

Read Glycoprotein Detection with the Odyssey Infrared Imaging System for more indepth information on using your Odyssey Infrared Imaging System for glycobiology research.

Image Studio Software from LI-COR^® is available for Mac^® and Windows^® computers. This intuitive software allows researchers to:

• Create individual work spaces
• Import tif, jpg, or png images from Odyssey, Pearl^®, or other imaging systems
• Image and export data
• Customize lab notebooks

Upgrade your current Odyssey or Pearl Imager software and all the benefits of this new, improved Image Studio Software.

If you would like to get an idea of how easy Image Studio Software is to use, try our free version – Image Studio Lite. Image Studio Lite allows you to analyze Western blot images but does not operate your LI-COR instrument to acquire images. Try it FREE – download now!

Click Chemistry utilizes pairs of reagents that exclusively react with each other and are effectively inert to naturally-occurring functional groups such as amines. Unlike affinity interactions such as streptavidin-biotin, Click Chemistry forges covalent bonds between the reacting partners to deliver stable bioconjugates.

Click Chemistry reactions can be categorized into two separate groups, copper-catalyzed or copper-free. Copper-catalyzed Click Chemistry is used for initiating reactions between azides and alkynes. These reactions are also known as Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC). Although they initiate and accelerate Click Reactions, copper catalysts are cytotoxic and inappropriate for use in living systems.

Watch this informative webinar on IRDye Infrared Dye Reagents for Click Chemistry.

With WesternSure ULTRA Chemiluminescent Substrate, picogram amounts of antigen can be detected. This substrate is optimized for detection using an imaging system or film.

For more sensitive detection – in the femtogram range – try WesternSure PREMIUM Chemiluminescent Substrate. The PREMIUM substrate is optimized for highly sensitive detection and long signal duration, making it ideal for use with imaging systems.

Ordering Information:

• WesternSure ULTRA Chemiluminescent Substrate, 100 mL kit, P/N 926-85000
• WesternSure PREMIUM Chemiluminescent Substrate, 100 mL kit, P/N 926-95000

Happy Blotting!

The dibenzocyclooctyne group (DBCO) allows copper-free Click Chemistry to be done with live cells, whole organisms, and non-living samples. DBCO groups will preferentially and spontaneously label molecules containing azide groups (—N3). Within physiological temperature and pH ranges, the DBCO group does not react with amines or hydroxyls, which are naturally present in many biomolecules. Reaction of the DBCO group with the azide group is significantly faster than with the sulfhydryl group (—SH, thiol).

These Click Chemistry products labeled with DBCO groups are available from LI-COR:

• IRDye 800CW DBCO Infrared Dye, 0.5 mg, P/N 929-50000
• IRDye 680RD DBCO Infrared Dye, 0.5 mg , P/N 929-50005
• IRDye 650 DBCO Infrared Dye, 0.5 mg, P/N 929-50010

Watch this 18-minute webinar to learn more about Click Chemistry applications and the new LI-COR^® Click Chemistry reagents.

BrightSite™ Optical Imaging Agents or probes developed using IRDye^® infrared dyes can be used for in vitro, in vivo, and tissue imaging. This technology offers researchers the ability to take research from the cell to the animal, all within one lab.

• Cell-based assays (binding capacity, specificity, competition, etc.) for optical agent development
• Histology and whole organ imaging for studying clearance and specificity
• Simultaneous two-color detection for two targets or one target with sample normalization

Pearl Impulse Small Animal Imaging Capabilities:

• Visualize biological processes in living animals with IRDye® optical agents
• Monitor multiple biological targets simultaneously
• Small, easy-to-use platform that is compatible with ready-to-use imaging agents—no transfections or probe development required

Figure 1. Validation and Use of an IRDye Fluorescent Probe. After probe labeling, in vitro cellular assays and microscopy are used to confirm specificity. The desired target is then imaged in animals. Excised organs and tissues can be examined for more detailed localization of the probe. Animal image captured with Pearl Impulse. A more comprehensive discussion of approaches for the development of fluorescent contrast agents has also been published. Reference: Kovar, et al. Anal Biochem 367(2007) 1-12.

Molecular imaging – achieved with near-infrared fluorescent technology from LI-COR!

Our innovative NEW Unicorn Secondary Antibodies for multicolor detection are exciting new additions to our line of IRDye^® infrared secondary antibodies. Feel the Magic!

Now, in addition the great imaging systems for chemiluminescent Western blots, LI-COR Biosciences offers chemiluminescent Western blotting substrates and HRP-conjugated secondary antibodies for use in performing your BEST chemiluminescent Western blots ever. And, the Glow-Writer™ Pen is a phosphorescent marker that can be used to annotate visible protein ladders for chemiluminescent Western detection.

WesternSure chemiluminescent reagents offer the best performance available when compared to other competitive products on the market. Both ULTRA and PREMIUM are highly sensitive enhanced substrates for detecting horseradish peroxidase (HRP) on immunoblots.

WesternSure HRP-conjugated secondary antibodies (Goat anti-Mouse and Goat anti-Rabbit) are compatible with a variety of chemiluminescent substrates and are optimized for use with WesternSure ULTRA and PREMIUM chemiluminescent substrates.

If you are in the US, check out the special March promotion for these New! Chemiluminescent Reagents (US and online orders only).

Happy Blotting!

FINALLY, you can image at your convenience. Keep your C-DiGit Blot Scanner on your lab bench, at your desk, or anywhere you choose (the image at the left shows just how small and portable the C-DiGit Scanner really is!).

The C-DiGit Chemiluminescent Western Blot Scanner maintains the simplicity of film exposures without the mess of the darkroom. You perform all of the same steps, without buying film and spending time in the darkroom. The C-DiGit Scanner gives you a complete digital replacement for film – keeping the advantages of film and eliminating many of the drawbacks – saving you time AND money!

Watch this short video and then go to mycdigit.com to get your very own C-DiGit Blot Scanner for only $4,995 (US List)!!

Just fill in the form and you will receive an email with a link to your Image Studio Lite download (Mac^® and Windows^® versions available). Watch the short Image Studio Lite tutorial videos and learn about all of the features and functions of Image Studio Lite.

With Image Studio Lite, you can:

• Import images from numerous sources
• Adjust brightness/contrast
• Define bands using shapes tools
• Obtain relative fluorescent units for shapes
• Export images and data
• Create lab notebooks

What can you do with Image Studio Lite? Find out in this short introductory video!