Tag Archives: chemiluminescent detection

WesternSure Chemiluminescent Substrates Offer Sensitive Detection of HRP on Western Blots

Are you doing chemiluminescent Western blots? If so, you should check out the new LI-COR chemiluminescent substrates. WesternSure chemiluminescent reagents are optimized for use with the C-DiGit™ Blot Scanner and the Odyssey® Fc Imaging System. These new chemiluminescent substrates offer … Continue reading

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NEW! WesternSure™ Chemiluminescent Western Blotting Reagents from LI-COR®

Detect your Western Blots with Confidence! Use NEW! WesternSure™ Chemiluminescent Reagents from LI-COR! Now, in addition the great imaging systems for chemiluminescent Western blots, LI-COR Biosciences offers chemiluminescent Western blotting substrates and HRP-conjugated secondary antibodies for use in performing your … Continue reading

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No Darkroom? No Problem! Get the LI-COR® C-DiGit™ Chemiluminescent Western Blot Scanner !

Are you doing chemiluminescent Western blots? Have you ever found yourself with a blot that is ready to image, but the darkroom is busy or the developer is broken? FINALLY, you can image at your convenience. Keep your C-DiGit Blot … Continue reading

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Western Blot Detection Methods: Fluorescence, Chemiluminescence, and Colorimetric?

Western blots can be detected with fluorescent, chemiluminescent, or colorimetric methods. What method do you use? Fluorescent detection: Fluorescent detection uses secondary antibodies labeled with fluorescent dyes, rather than enzymes. No substrates are needed. Enzymatic detection: Chemiluminescent and colorimetric methods … Continue reading

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LI-COR® Odyssey® Imagers: The Standard for Quantitative Western Blotting

As a researcher, your goal is to efficiently present the most accurate data possible. For more than 30 years, traditional chemiluminescent detection with film has provided data that have been published by scientists worldwide. Over the past decade, LI-COR has … Continue reading

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Quantitative Cell Signaling Analysis – What’s all the Buzz About?

What’s all this BUZZZZ you are hearing about being able to quantitate cell signaling in plate-based assays? If you are at AACR in Chicago this week, stop by Booth 3800 (LI-COR® Biosciences) and we can tell you all about the … Continue reading

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Digital Chemiluminescent Western Blots – Substrate Selection is Critical

So, you’ve selected your primary antibodies with care (see Know Thy Primary Antibody), and you’re using a great HRP-conjugated secondary antibodies. NOW – what about the chemiluminescent substrate? Yes, I know, there are tons of different substrates and vendors out … Continue reading

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Love Traditional Western Blotting with Chemiluminescence Detection? Go Digital!

Tired of the darkroom being down or the expense of the development chemicals? Oh, AND all that film you go through because you have to do multiple exposures to get the image just right? Go DIGITAL with the Odyssey® Fc … Continue reading

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Chemiluminescent Western Blots – HRP-Conjugated Secondary Antibody Selection

So we’ve talked about choosing primary antibodies , and you have come to know the ones you are using very well. We have also walked through some frequently asked questions about primary and secondary antibodies for chemi Westerns. The next … Continue reading

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Chemiluminescent Western Blots – FAQs about Primary and Secondary Antibodies

We’ve discussed some hints on how there can be considerable difference in primary antibodies – so “Know thy Primary Antibody.“ In addition, we’ve received some questions that are frequently asked – hence called frequently asked questions or FAQs – about … Continue reading

Posted in IRDye Reagents, Odyssey Imaging Systems, Proteomics Applications, Western Blotting | Tagged , , , , , , , , | Leave a comment