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Tag Archives: detecting large proteins
Troubleshooting In-Gel Westerns – Where’s the Signal?
Okay, so you’re doing an in-gel western because you have a hard-to-transfer target (say, a glycoprotein). And you are using near-infrared fluorescence detection because it gets rid of inconsistencies due to transfer (and with an Odyssey it’s really fast and … Continue reading
Posted in Odyssey Imaging Systems, Proteomics Applications, Western Blotting
Tagged detecting large proteins, in gel westerns, li-cor western blotting, licor western blotting, licor westerns, near-infrared fluorescent reagents, odyssey applications, western blot troubleshooting, western blots, western blotting
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Troubleshooting In-Gel Westerns – What can cause High Background?
So, you are doing an in-gel western because you have a difficult to transfer protein. Good for you!! But, you are seeing high background – and now you need some help to optimize your application. What causes high background on … Continue reading
Optimizing your Near-infrared Fluorescent In-Gel Western
A Powerful Technique for Large, Hard-to-transfer Proteins The In-Gel Western detection protocol may require optimization for each target protein or gel type. Sensitivity of In-Gel Westerns may be lower than standard Western blots. (Transfer to a membrane concentrates the target … Continue reading
In-Gel Westerns on the Odyssey® CLx or Classic – Difficult Proteins Detected More Easily
Western blot detection of proteins requires separation of protein mixtures by electrophoresis, followed by transfer of the separated proteins to nitrocellulose or PVDF membranes for detection. In-Gel Western detection avoids transfer problems by directly detecting target proteins within the polyacrylamide … Continue reading
