Categories
Archives
Links
Tag Cloud
Tag Archives: ecl detection
Digital Chemiluminescent Western Blots – Substrate Selection is Critical
So, you’ve selected your primary antibodies with care (see Know Thy Primary Antibody), and you’re using a great HRP-conjugated secondary antibodies. NOW – what about the chemiluminescent substrate? Yes, I know, there are tons of different substrates and vendors out … Continue reading
Posted in Odyssey Imaging Systems, Proteomics Applications, Western Blotting
Tagged chemi westerns, chemiluminescent detection, chemiluminescent substrates, ecl detection, li-cor western blots, li-cor western blotting, licor odyssey fc, licor western blotting, licor westerns, odyssey applications, odyssey fc, western blot troubleshooting, western blots, western blotting
Leave a comment
Love Traditional Western Blotting with Chemiluminescence Detection? Go Digital!
Tired of the darkroom being down or the expense of the development chemicals? Oh, AND all that film you go through because you have to do multiple exposures to get the image just right? Go DIGITAL with the Odyssey® Fc … Continue reading
Chemiluminescent Western Blots – HRP-Conjugated Secondary Antibody Selection
So we’ve talked about choosing primary antibodies , and you have come to know the ones you are using very well. We have also walked through some frequently asked questions about primary and secondary antibodies for chemi Westerns. The next … Continue reading
Posted in Odyssey Imaging Systems, Proteomics Applications, Western Blotting
Tagged chemi westerns, chemiluminescent detection, chemiluminescent western blots, ecl detection, li-cor western blots, li-cor western blotting, licor odyssey fc, licor westerns, odyssey fc, secondary antibodies, western blot troubleshooting, western blots, western blotting
Leave a comment
Chemiluminescent Western Blots – FAQs about Primary and Secondary Antibodies
We’ve discussed some hints on how there can be considerable difference in primary antibodies – so “Know thy Primary Antibody.“ In addition, we’ve received some questions that are frequently asked – hence called frequently asked questions or FAQs – about … Continue reading
Posted in IRDye Reagents, Odyssey Imaging Systems, Proteomics Applications, Western Blotting
Tagged chemi westerns, chemiluminescent detection, ecl detection, li-cor western blots, li-cor western blotting, licor odyssey fc, primary antibodies, western blot troubleshooting, western blotting
Leave a comment
Optimizing Chemiluminescent Western Blots – The Best Offense is a Good Blocker
Okay, it’s football season, and I thought the analogy fit. Seriously, the right Blocking Buffer is critical to getting that great chemiluminescent Western blot. Incubating the membrane in blocking buffer after the transfer step will result in enhanced sensitivity of … Continue reading
