If you are doing flow cytometry or microscopy and need dye-labeled secondary antibodies in the visible fluorescence range, we can help. LI-COR now offers IRDye® and VRDye™ dye-labeled secondary antibodies for 650nm, 549nm, and 490nm detection.
VRDye secondary antibodies are are highly cross-adsorbed – just like our IRDye secondary antibodies, making them suitable for multi-color detection.
Here is an example of immunofluorescence staining using VRDye 490 Goat anti-Rabbit Secondary Antibody.
Immunofluorescence staining of tubulin protein in HeLa cells. Cells were cultured on cover slips. After fixation and permeabilization, cells were incubated with rabbit anti-tubulin mAb (CST), followed by VRDye 490 Goat anti-Rabbit IgG (P/N 926-49020). Nuclei were stained with DAPI. Image acquired with Olympus IX81 microscope.
Are you ready to try IRDye Infrared Dyes and secondary conjugates or VRDye visible fluorescent secondaries on your epifluorescent microscope? Check out the recommended configurations for Olympus and Zeiss microscopes – and go image!