5 Technical Tips for Chemiluminescent Western Blotting Success with the C-DiGit® Scanner

In this short video, Jessica talks about 5 tips to help ensure that imaging chemiluminescent Western blots on the C-DiGit Blot Scanner is a success – the first time and always!

Here’s a recap of the tips of those five technical tips:

  1. Install Image Studio™ Software on your computer before connecting the C-DiGit Blot Scanner.
  2. Incubate using room temperature substrate.
  3. Wrap your blot so it stays wet during the scan.
    1. Remember to place your blot protein side down.
    2. If sensitivity is an issue, use WesternSure® PREMIUM or SuperSignal® West Femto Chemiluminescent Substrate.
  4. Start with high sensitivity scan for your first scan and then work from there.
  5. Image on the C-DiGit Scanner first and then exposure your film.

C-DiGit Blot ScannerHappy Blotting!

Easy-to-Use Image Studio v3.1 Software Now Available for all Odyssey® and Pearl® Imaging Systems



Image Studio™ Software
from LI-COR® is available for Mac® and Windows® computers. This intuitive, easy-to-use software allows researchers to:

  • Create individual work spaces
  • Import tif, jpg, or png images from Odyssey, Pearl®, or other imaging systems
  • Image and export data
  • Customize lab notebooks

Upgrade your current Odyssey or Pearl Imager software and all the benefits of this new, improved easy-to-use Image Studio Software.

If you would like to get an idea of how easy Image Studio Software is to use, try our free version – Image Studio Lite. Image Studio Lite allows you to analyze Western blot images but does not operate your LI-COR instrument to acquire images. Try it FREE – download now! Then, tell your friends – they are sure to want a copy, too!