BrightSite™ Optical Imaging Agents or probes developed using IRDye® infrared dyes can be used for in vitro, in vivo, and tissue imaging. This technology offers researchers the ability to take research from the cell to the animal, all within one lab.
Odyssey CLx Infrared Imaging System Capabilities:
- Cell-based assays (binding capacity, specificity, competition, etc.) for optical agent development
- Histology and whole organ imaging for studying clearance and specificity
- Simultaneous two-color detection for two targets or one target with sample normalization
Pearl Small Animal Imaging Capabilities:
- Visualize biological processes in living animals with IRDye® optical agents
- Monitor multiple biological targets simultaneously
- Small, easy-to-use platform that is compatible with ready-to-use imaging agents—no transfections or probe development required
Figure 1. Validation and Use of an IRDye Fluorescent Probe. After probe labeling, in vitro cellular assays and microscopy are used to confirm specificity. The desired target is then imaged in animals. Excised organs and tissues> can be examined for more detailed localization of the probe. Animal image captured with Pearl Imaging System. A more comprehensive discussion of approaches for the development of fluorescent contrast agents has also been published. Reference: Kovar, et al. Anal Biochem 367(2007) 1-12.