Tag Archives: licor western blotting

WesternSure Chemiluminescent Substrates Offer Sensitive Detection of HRP on Western Blots

Are you doing chemiluminescent Western blots? If so, you should check out the new LI-COR chemiluminescent substrates. WesternSure chemiluminescent reagents are optimized for use with the C-DiGit™ Blot Scanner and the Odyssey® Fc Imaging System. These new chemiluminescent substrates offer … Continue reading

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NEW! WesternSure™ Chemiluminescent Western Blotting Reagents from LI-COR®

Detect your Western Blots with Confidence! Use NEW! WesternSure™ Chemiluminescent Reagents from LI-COR! Now, in addition the great imaging systems for chemiluminescent Western blots, LI-COR Biosciences offers chemiluminescent Western blotting substrates and HRP-conjugated secondary antibodies for use in performing your … Continue reading

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No Darkroom? No Problem! Get the LI-COR® C-DiGit™ Chemiluminescent Western Blot Scanner !

Are you doing chemiluminescent Western blots? Have you ever found yourself with a blot that is ready to image, but the darkroom is busy or the developer is broken? FINALLY, you can image at your convenience. Keep your C-DiGit Blot … Continue reading

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NEW! IRDye® Goat Anti-Mouse IgM Secondary Antibodies from LI-COR®!

Our IRDye secondary antibody line is growing! We have recently added IRDye Goat anti-Mouse IgM (μ chain specific) secondaries labeled with: IRDye 800CW (PN 926-32280) IRDye 680RD (PN 926-68180) or IRDye 680LT (PN 926-68080). Just like all of the LI-COR … Continue reading

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Odyssey® Sa: the Economical Imager Solution for Quantitative IR Westerns and Plate-based Assays

Is your capital equipment budget money tight? (when isn’t it, right?) Well, if you want to do quantitative infrared Western blotting AND plate-based assays, you should consider the economical Odyssey Sa Infrared Imaging System. Less expensive than the multi-functional, supports-more-than-20 … Continue reading

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Reprobe Fluorescent Westerns, Conserve Precious Samples with NewBlot™ Stripping Buffer

NewBlot Western Blot Stripping Buffer is specially formulated for use with IRDye® infrared dyes (680 and 800CW only) and the Odyssey® Infrared Imaging Systems to help you save time and money on recreating samples. NewBlot Stripping Buffer allows you to … Continue reading

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Western Blot Detection Methods: Fluorescence, Chemiluminescence, and Colorimetric?

Western blots can be detected with fluorescent, chemiluminescent, or colorimetric methods. What method do you use? Fluorescent detection: Fluorescent detection uses secondary antibodies labeled with fluorescent dyes, rather than enzymes. No substrates are needed. Enzymatic detection: Chemiluminescent and colorimetric methods … Continue reading

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Monitor Protein Levels and Phosphorylation with Quantitative Multiplexed Western Blots

Molecular profiling reveals diversity of stress signal transduction cascades in highly penetrant Alzheimer’s disease human skin fibroblasts. Mendonsa, G., et al. PLoS ONE 4(2): e4655. doi:10.1371/journal.pone.0004655 Aberrant signal transduction is associated with Alzheimer’s disease (AD). In skin fibroblasts of AD … Continue reading

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LI-COR® Odyssey® Imagers: The Standard for Quantitative Western Blotting

As a researcher, your goal is to efficiently present the most accurate data possible. For more than 30 years, traditional chemiluminescent detection with film has provided data that have been published by scientists worldwide. Over the past decade, LI-COR has … Continue reading

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Troubleshooting In-Gel Westerns – Where’s the Signal?

Okay, so you’re doing an in-gel western because you have a hard-to-transfer target (say, a glycoprotein). And you are using near-infrared fluorescence detection because it gets rid of inconsistencies due to transfer (and with an Odyssey it’s really fast and … Continue reading

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