Multiplex Western Blotting System Turbo-Charges Western Blot Results Output

Example of Multiplex Western Blotting using the MPX Blotting SystemMultiplex Western blotting is a powerful tool that allows you to get more out of your Western blots. Multiplex detection becomes possible when you utilize the MPX™ (Multiplex) Blotting System and LI-COR IRDye® near-infrared fluorescent dye-labeled secondary antibodies.

Multiplex Westerns can be imaged on any of the Odyssey® Imagers and provide results for a possible maximum of 48 targets on a single membrane — 24 per channel with two-color detection — and the option for quantitative analysis, saving you time and reagents! The MPX Blotting System can be used if you need to optimize:

  • Primary antibodies – to determine the primary antibody that has the right specificity and the right dilution for use
  • Antibody incubation times
  • Blocking conditions – which blocking buffer will give you the optimum results
  • Secondary antibodies – what dilutions is best to use without getting a lot of non-specific binding?
  • Or just about anything else you need to optimize!

Watch this 4 minute video on how easy it is to get the most out of multiplexing with the MPX Blotting System. You can also download the handy MPX Blotter User Guide.

Odyssey® References for Neuroscience Research Applications

Otis Spunkmeyer Chocolate Chip Cookie Odyssey Infrared Imaging Systems and LI-COR® reagents have been used for neuroscience research applications in many different disciplines, including neurobiology, Alzheimer’s Disease, Parkinson’s, neural tumors, and more.

This week the Society for Neuroscience is holding its annual meeting in New Orleans, LA. LI-COR is there showcasing the Odyssey CLx and the Odyssey Fc, plus the MPX™ Multiplex Blotting System, and several new products. Stop by Booth 2713 and talk to our LI-COR representatives. Fresh chocolate chip cookies will be available Monday and Tuesday at the booth during certain times – while supplies last! (follow us on Twitter – @licorbio – to find out when to stop by and get a fresh cookie snack!)
LI-COR Brain Pen

Plus pick up your free ‘brain’ pen at the LI-COR Booth (#2713).

Enjoy the conference! We wish you continued success in your research!

Here are a few journal references related to research in the Neurosciences:

Conditional Deletion of Notch1 and Notch2 Genes in Excitatory Neurons of Postnatal Forebrain Does Not Cause Neurodegeneration or Reduction of Notch mRNAs and Proteins
Jin Zheng, Hirotaka Watanabe, Mary Wines-Samuelson, Huailong Zhao, Thomas Gridley, Raphael Kopan, and Jie Shen
J. Biol. Chem., Jun 2012; 287: 20356 – 20368.

Enhancement of Rostral Ventrolateral Medulla Neuronal Nitric-Oxide Synthase–Nitric-Oxide Signaling Mediates the Central Cannabinoid Receptor 1-Evoked Pressor Response in Conscious Rats
Badr Mostafa Ibrahim and Abdel A. Abdel-Rahman
J. Pharmacol. Exp. Ther., Jun 2012; 341: 579 – 586.

Specific Serine-Proline Phosphorylation and Glycogen Synthase Kinase 3β-directed Subcellular Targeting of Stathmin 3/Sclip in Neurons
Sara Devaux, Fabienne E. Poulain, Véronique Devignot, Sylvie Lachkar, Theano Irinopoulou, and André Sobel
J. Biol. Chem., Jun 2012; 287: 22341 – 22353.

For more journal references citing the use of the Odyssey Imagers and LI-COR reagents, see our most recent Publications Lists.