Tag Archives: near-infrared fluorescent reagents

NEW! IRDye® Goat Anti-Mouse IgM Secondary Antibodies from LI-COR®!

Posted on November 29, 2012 by licor-admin

Our IRDye secondary antibody line is growing! We have recently added IRDye Goat anti-Mouse IgM (μ chain specific) secondaries labeled with: IRDye 800CW (PN 926-32280) IRDye 680RD (PN 926-68180) or IRDye 680LT (PN 926-68080). Just like all of the LI-COR … Continue reading →

Posted in IRDye Reagents, Proteomics Applications, Reagents, Western Blotting | Tagged , , , , , , , , , , , , , , , | Leave a comment

New! Optical Probe for Tumor Imaging – IRDye® 800CW YC-27

Posted on October 3, 2012 by licor-admin

IRDye 800CW YC-27 (P/N 926-27000) is a near-infrared dye-labeled imaging agent specifically designed to target prostate specific membrane antigen (PSMA), also known as folate hydrolase I or glutamate carboxypeptidase II. This small molecule can be used as an optical imaging … Continue reading →

Posted in Cancer Research, IRDye Reagents, In Vivo Imaging System and Applications, Reagents | Tagged , , , , , , , , , , , , , | Leave a comment

Monitor Protein Levels and Phosphorylation with Quantitative Multiplexed Western Blots

Posted on August 10, 2012 by licor-admin

Molecular profiling reveals diversity of stress signal transduction cascades in highly penetrant Alzheimer’s disease human skin fibroblasts. Mendonsa, G., et al. PLoS ONE 4(2): e4655. doi:10.1371/journal.pone.0004655 Aberrant signal transduction is associated with Alzheimer’s disease (AD). In skin fibroblasts of AD … Continue reading →

Posted in Neuroscience, Odyssey Imaging Systems, Western Blotting | Tagged , , , , , , , , , , , , | Leave a comment

Normalize Protein Concentrations with Nucleic Acid Stains in In-Cell Western™ Assays

Posted on June 25, 2012 by licor-admin

When performing the In-Cell Western assay, one fluorescence channel is often used for normalization. This allows quantification data for the protein of interest to be corrected for well-to-well variation in cell number, thereby increasing the overall accuracy of the assay. … Continue reading →

Posted in Cancer Research, Proteomics Applications | Tagged , , , , , , , | Leave a comment

In-Cell Western™ Assay Application: Response of COS-7 Cells to Hydroxyurea

Posted on June 15, 2012 by licor-admin

Application: Detecting phospho-p53 in COS cells in response to Hydroxyurea The response of COS-7 cells to increasing doses of hydroxyurea was measured by a specific antibody (Anti-phospho-p53 from Cell Signaling Technology, P/N 9286) that detects phosphorylated-p53 (Ser16). Total ERK1 was … Continue reading →

Posted in Cancer Research, IRDye Reagents, Odyssey Imaging Systems, Proteomics Applications | Tagged , , , , , , , | Leave a comment

Suspension Cells for Quantitative Cell Signaling Analysis using In-Cell Western™ Assays

Posted on May 31, 2012 by licor-admin

So I’ve talked about how to ensure that suspension cells attach to plates, when to know you have a monolayer, and why round bottom plates are the best when doing In-Cell Western Assays with suspension cells in the 23-May-12 blog … Continue reading →

Posted in Odyssey Imaging Systems, Proteomics Applications | Tagged , , , , , , , , , | Leave a comment

Washing Gently and Round Bottom Plates are Essential for Successful In-Cell ELISAs Using Non-Adherent Cells

Posted on May 29, 2012 by licor-admin

I mentioned in my post on 23-May, that the next few entries would be on more hints and tips of how to use non-adherent cells for In-Cell Western Assays – so here goes! During my washing steps, cells are coming … Continue reading →

Posted in Odyssey Imaging Systems, Proteomics Applications | Tagged , , , , , , , , , , , | Leave a comment

Using Non-adherent Cells for Fluorescent Immunoassays – Tips for Successful In-Cell Western™ Assays

Posted on May 23, 2012 by licor-admin

You might be wondering if this powerful technique called In-Cell Western Assay can be used for your cell line because your cell line is non-adherent. Well, you are in luck! You CAN use suspension cells for ICW Assays – with … Continue reading →

Posted in Odyssey Imaging Systems, Proteomics Applications | Tagged , , , , , , , , , , , | Leave a comment

Receptor-based Binding and Competition Assays with Infrared Fluorescence

Posted on May 15, 2012 by licor-admin

What is an On-Cell Western Assay? It’s a cell-based assay that enables quantitative monitoring of cell surface protein expression. The On-Cell Western assay offers the ability to: Detect and quantify target proteins localized to the cell surface Quantify ligand binding … Continue reading →

Posted in Neuroscience, Odyssey Imaging Systems, Proteomics Applications | Tagged , , , , , , , , , | Leave a comment

Fast, Cost-Effective Cell-Based Assays for Quantitative Cell Signaling Analysis

Posted on April 27, 2012 by licor-admin

The In-Cell Western™ Assay is an immunocytochemical assay that uses near-infrared fluorescence to detect and quantify proteins in fixed cells. Detecting proteins in their cellular context increases quantification precision. Proteins in fixed, cultured cells are detected directly in microplates, which … Continue reading →

Posted in Odyssey Imaging Systems, Proteomics Applications | Tagged , , , , , , , , , , | Leave a comment