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Tag Archives: odyssey applications
Rethinking the Traditional Western Blot
Traditional Western blotting is a labor-intensive process that includes gel electrophoresis, protein transfer to a blotting membrane, incubation with primary and secondary antibodies, and chemiluminescent or fluorescent detection of target proteins. (View a typical Western blotting workflow.) Day-to-day reproducibility is … Continue reading
Posted in Neuroscience, Odyssey Imaging Systems, Proteomics Applications, Reagents
Tagged alzheimers research, cell based assays, cell-based ELISA, chad dickey research, cytoblots, dickey paper on alzheimers, fluorescent immunoassays, icw applications, ICW assays, icw webinar, in cell westerns, in-cell ELISA, In-Cell Western assays, neurobiology, normalization of proteins, odyssey applications, plate-based assays, protein quantification, tau protein accumulation, taw protein inhibition
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New Cell Stain Increases Ease of Use for In-Cell Western™ Normalization
Have you ever wanted to try an in-cell ELISA but you just weren’t sure how to get started? With the new LI-COR® CellTag™ 700 Stain, a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number, you have … Continue reading
Posted in Proteomics Applications, Reagents
Tagged cell stain, celltag 700 stain, celltag kits, celltag stain, ICW assays, icw kits, in cell westerns, in-cell ELISA, In-Cell Western assays, in-cell western kits, near infrared fluorescent stain, non-specific cell stain, normalization stain, odyssey applications
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Journal Articles Citing Use of Odyssey® and Pearl® Imaging Systems and Near-Infrared Fluorescence
Affibody-DyLight Conjugates for in vivo Assessment of HER2 Expression by Near-Infrared Optical Imaging. Zielinski R, M Hassan, I Lyakhov, D Needle, V Chernomordik, A Garcia-Glaessner, Y Ardeshirpour, J Capala and A Gandjbakhche Radiation Oncology Branch, Center for Cancer Research, National … Continue reading
Posted in Cancer Research, IRDye Reagents, In Vivo Imaging System and Applications, Odyssey Imaging Systems, Western Blotting
Tagged apoptosis, biomarkers for cancer, breast cancer research, ca125, cesicular trafficking, chronic hypoxia, egfr, egfr inhibitors, glycobiology, glycoprotein analysis, her2-specific probes, hypoxia, in vivo imaging, molecular imaging, n-linked glycoproteins, odyssey applications, optical imaging, ovarian cancer research, pearl applications, potassium channels, protein quantitation, publication lists, quantative western blots, quantitative ir western blots, quantitative proteomics, quantitative western blotting
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Create a Complete Molecular Imaging Workstation
Combining the Odyssey® CLx Infrared Imaging System with the Pearl® Impulse Small Animal Imaging System creates a versatile workstation for in vivo and in vitro imaging. BrightSite™ Optical Imaging Agents or probes developed using IRDye® infrared dyes can be used … Continue reading
Posted in IRDye Reagents, In Vivo Imaging System and Applications, Odyssey Imaging Systems
Tagged brightsite imaging agents, in vitro assays, in vivo imaging, in vivo imaging workstation, infrared dyes, irdye optical probes, molecular imaging, odyssey applications, pearl applications, pearl impulse small animal imaging system, preclinical research, probe validation, small animal imaging, workstation
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LI-COR® Odyssey® Imagers: The Standard for Quantitative Western Blotting
As a researcher, your goal is to efficiently present the most accurate data possible. For more than 30 years, traditional chemiluminescent detection with film has provided data that have been published by scientists worldwide. Over the past decade, LI-COR has … Continue reading
In-Cell Western™ Assay Application: Response of COS-7 Cells to Hydroxyurea
Application: Detecting phospho-p53 in COS cells in response to Hydroxyurea The response of COS-7 cells to increasing doses of hydroxyurea was measured by a specific antibody (Anti-phospho-p53 from Cell Signaling Technology, P/N 9286) that detects phosphorylated-p53 (Ser16). Total ERK1 was … Continue reading
Posted in Cancer Research, IRDye Reagents, Odyssey Imaging Systems, Proteomics Applications
Tagged cell based assays, immunofluorescent assays, in-cell ELISA, licor odyssey imagers, near-infrared fluorescent reagents, odyssey applications, protein phosphorylation, signal transduction
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Suspension Cells for Quantitative Cell Signaling Analysis using In-Cell Western™ Assays
So I’ve talked about how to ensure that suspension cells attach to plates, when to know you have a monolayer, and why round bottom plates are the best when doing In-Cell Western Assays with suspension cells in the 23-May-12 blog … Continue reading
Posted in Odyssey Imaging Systems, Proteomics Applications
Tagged apoptosis, cell based assays, immunofluorescent assays, in-cell ELISA, licor odyssey imagers, near-infrared fluorescent reagents, odyssey applications, signal transduction, using cells in suspension, using non-adherent cells in plate-based assays
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Washing Gently and Round Bottom Plates are Essential for Successful In-Cell ELISAs Using Non-Adherent Cells
I mentioned in my post on 23-May, that the next few entries would be on more hints and tips of how to use non-adherent cells for In-Cell Western Assays – so here goes! During my washing steps, cells are coming … Continue reading
Posted in Odyssey Imaging Systems, Proteomics Applications
Tagged cell based assays, ICE assays, ICW assays, in-cell ELISA, near-infrared fluorescent reagents, odyssey applications, plate-based assays, signal transduction, using cells in suspension, using non-adherent cells in plate-based assays, using nonadherent cells for icw, using suspension cells
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