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Tag Archives: western blotting
WesternSure Chemiluminescent Substrates Offer Sensitive Detection of HRP on Western Blots
Are you doing chemiluminescent Western blots? If so, you should check out the new LI-COR chemiluminescent substrates. WesternSure chemiluminescent reagents are optimized for use with the C-DiGit™ Blot Scanner and the Odyssey® Fc Imaging System. These new chemiluminescent substrates offer … Continue reading
Posted in Reagents, Western Blotting
Tagged chemi westerns, chemiluminescent detection, chemiluminescent substrates, chemiluminescent western blots, detect femtograms on western blot, detect picograms on western blot, hrp conjugated antibodies, immunoblots, li-cor western blots, li-cor western blotting, licor western blotting, licor westerns, western blotting, westernsure premium, westernsure substrates, westernsure ultra
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NEW! WesternSure™ Chemiluminescent Western Blotting Reagents from LI-COR®
Detect your Western Blots with Confidence! Use NEW! WesternSure™ Chemiluminescent Reagents from LI-COR! Now, in addition the great imaging systems for chemiluminescent Western blots, LI-COR Biosciences offers chemiluminescent Western blotting substrates and HRP-conjugated secondary antibodies for use in performing your … Continue reading
Posted in Reagents, Western Blotting
Tagged chemi westerns, chemiluminescent detection, chemiluminescent substrates, chemiluminescent western blots, chemiluminescent western blotting, hrp conjugated secondaries, hrp conjugted secondary antibodies, li-cor western blots, li-cor western blotting, licor chemiluminescent substrates, licor western blotting, licor westerns, marker pen for chemiluminescent western blots, reagents for chemiluminescence western detection, western blots, western blotting, westernsure reagents
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Coming in 2013! LI-COR® C-DiGit™ System – Digital Film for Chemiluminescent Western Blot Imaging
Are you tired of waiting in line for the darkroom? Or spending your precious budget monies on all that film? OR having to do multiple exposures to get just the right image for publication? Then, you need DIGITAL FILM! But … Continue reading
Posted in C-DiGit System, Western Blotting
Tagged c-digit system, chemi imager, chemi westerns, chemiluminescence detection, chemiluminescent western blot imaging, chemiluminescent western blots, digital film, digital imaging, licor c-digit, licor cdigit, licor westerns, western blotting
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Western Blot Detection Methods: Fluorescence, Chemiluminescence, and Colorimetric?
Western blots can be detected with fluorescent, chemiluminescent, or colorimetric methods. What method do you use? Fluorescent detection: Fluorescent detection uses secondary antibodies labeled with fluorescent dyes, rather than enzymes. No substrates are needed. Enzymatic detection: Chemiluminescent and colorimetric methods … Continue reading
NEW! COX IV Primary Antibody Offers Normalization for Low-Expressing Proteins
The COX IV primary antibody can be used for detection of COX IV by Western blot, or as a normalization antibody when performing two-color detection. Its target molecular weight is 17 kDa. Detection of this primary antibody can be achieved … Continue reading
Posted in Proteomics Applications, Reagents, Western Blotting
Tagged antibodies for normalization, cox iv antibody, how to normalize Western blots, immunofluorescent assays, in-cell ELISA, li-cor western blots, li-cor western blotting, licor odyssey imagers, normalization, primary antibodies, primary antibody, western blotting
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LI-COR® Odyssey® Imagers: The Standard for Quantitative Western Blotting
As a researcher, your goal is to efficiently present the most accurate data possible. For more than 30 years, traditional chemiluminescent detection with film has provided data that have been published by scientists worldwide. Over the past decade, LI-COR has … Continue reading
Troubleshooting In-Gel Westerns – Where’s the Signal?
Okay, so you’re doing an in-gel western because you have a hard-to-transfer target (say, a glycoprotein). And you are using near-infrared fluorescence detection because it gets rid of inconsistencies due to transfer (and with an Odyssey it’s really fast and … Continue reading
Posted in Odyssey Imaging Systems, Proteomics Applications, Western Blotting
Tagged detecting large proteins, in gel westerns, li-cor western blotting, licor western blotting, licor westerns, near-infrared fluorescent reagents, odyssey applications, western blot troubleshooting, western blots, western blotting
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Digital Chemiluminescent Western Blots – Substrate Selection is Critical
So, you’ve selected your primary antibodies with care (see Know Thy Primary Antibody), and you’re using a great HRP-conjugated secondary antibodies. NOW – what about the chemiluminescent substrate? Yes, I know, there are tons of different substrates and vendors out … Continue reading
Posted in Odyssey Imaging Systems, Proteomics Applications, Western Blotting
Tagged chemi westerns, chemiluminescent detection, chemiluminescent substrates, ecl detection, li-cor western blots, li-cor western blotting, licor odyssey fc, licor western blotting, licor westerns, odyssey applications, odyssey fc, western blot troubleshooting, western blots, western blotting
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Would you like to save at least 90 minutes the next time you do a Western blot?
The Quick Western Kit — IRDye® 680RD (P/N 926-68100) provides a universal detection reagent that can be combined with the primary antibody incubation step, eliminating the need for a secondary antibody incubation step. This kit works with a variety of … Continue reading
Posted in IRDye Reagents, Odyssey Imaging Systems, Proteomics Applications, Western Blotting
Tagged li-cor western blots, li-cor western blotting, licor odyssey imagers, licor western blotting, near-infrared fluorescent reagents, western blots, western blotting, Western blotting kits
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Scientists using Quantitative Western blotting and Odyssey® Infrared Imagers
There are over 4000 peer-reviewed journal articles in which scientists have cited the use of LI-COR products and imaging systems for all types of research – from apoptosis, autophagy, and angiogenesis to RNAi studies, transcription factor assays, and virology – … Continue reading
Posted in Odyssey Imaging Systems, Proteomics Applications, Western Blotting
Tagged angiogenesis, apoptosis, autophagy, cell based assays, li-cor western blotting, near-infrared fluorescent reagents, odyssey applications, RNAi studies, transcription factors, virology, western blotting
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