Scientists using Quantitative Western blotting and Odyssey® Infrared Imagers

The Odyssey Family of Imaging SystemsThere are over 4000 peer-reviewed journal articles in which scientists have cited the use of LI-COR products and imaging systems for all types of research – from apoptosis, autophagy, and angiogenesis to RNAi studies, transcription factor assays, and virology – and many disciplines inbetween.

Here is a review of a recent publication in which quantitative Western blots were performed on the Odyssey Infrared Imaging System.

High-Content Chemical and RNAi Screens for Suppressors of Neurotoxicity in a Huntington’s Disease Model

Joost Schulte, Katharine J. Sepp, Chaohong Wu, Pengyu Hong, J. Troy Littleton
Dept of Biology, Dept of Brain and Cognitive Sciences, The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA

PLoS ONE 6(8): e23841 (2011)

Huntington’s Disease (HD), a dominant neurodegenerative disorder, results from expansion of a polyglutamine (polyQ) tract in the Huntingtin (Htt) protein. This study describes a high-content small molecule and RNAi screen for suppressors of neurotoxicity, using a Drosophila primary neural culture HD model. An mRFP-tagged pathogenic Huntingtin variant (Htt138Q) was expressed to induce disease phenotypes. Suppressors of neurotoxicity were identified, including lkb1 (an upstream kinase in the mTOR/insulin pathway) and four drugs (Camptothecin, OH-Camptothecin, 18β-Glycyrrhetinic acid, and Carbenoxolone). Quantitative Western blotting with the Odyssey Imager was used to monitor expression of Htt variants, and for in vivo validation of screen hits. The suppressors identified in this screen also restored viability in an in vivo Drosophila HD model.

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Updated November 20, 2017.