Highlighted Publications

Proia, D. A., et al.
Multifaceted Intervention by the Hsp90 Inhibitor Ganetespib (STA-9090) in Cancer Cells with Activated JAK/STAT Signaling
PLoS ONE 6:  e18552 (April 2011)

Proia et al. used quantitiative Westerns on the Odyssey Imager to show that the novel small molecule Hsp90 inhibitor ganetespib (formerly STA-9090) exhibits potent in vitro and in vivo activity in a range of solid and hematological tumor cells that are dependent on JAK2 activity for growth and survival.

Rubbi, L., et al.
Global Phosphoproteomics Reveals Crosstalk Between Bcr-Abl and Negative Feedback Mechanisms Controlling Src Signaling
Science Signaling 4: 1 (March 29, 2011).

In leukemias driven by the tyrosine kinase fusion protein Bcr-Abl, signaling by the Src family kinases (SFKs) critically contributes to the leukemic phenotype. Rubbi et al. performed global tyrosine phosphoprofiling by quantitative mass spectrometry of Bcr-Abl–transformed cells in which the activities of the SFKs were perturbed, to build a detailed context-dependent network of cancer signaling. Quantitative Western blotting on Odyssey showed rapid decay of the Src Y 416 activating phosphorylation that matched the kinetics of overall Tyr phosphorylation, whereas phosphorylation of the C-terminal negative regulatory phosphorylation (as measured by antibodies against Lyn Y 507 and Src Y 527) did not decay.

Skinner, H. D., et al.
Disruptive Mutations Lead to Head and Neck Cancer Treatment Failure through Inhibition of Radiation-Induced Senescence
Clin Cancer Res 18: 290 – 300 (Jan 2012).

Larance, M., et al.
Characterization of MRFAP1 Turnover and Interactions Downstream of the NEDD8 Pathway
Mol Cell Proteomics 11: M111.014407 (Mar 2012).