GEMF Costs & Equipment

Things to Consider When Buying a System.

To help you estimate on-going costs associated with using the LI-COR 4300 System in your classroom, we have prepared the following guides. Please download the PDF guide(s) for the applications you plan to use. These guides and other GEMF resources are also available on our Undergraduate Support page in our Biolibrary.

• AFLP® Analysis
• Gel Chemistry
• Microsatellite Analysis
• Sequencing

Once you've decided on a course plan, you'll need to outline the equipment you need for your classroom. An example of an equipment checklist is provided below. You will need a list of equipment when you apply for a grant.

LI-COR System Features Make a Perfect Fit for Student Research.

• The system is rugged, reliable, and easy to set up and operate by students.

• LI-COR has features that make it a perfect fit for student research (gel image, complete range of protocols with primer and acycloterminator labeling, pipetting and reagent measurement).

• System is suited to teach students sequencing theory, image interpretation, and curve analysis.

• Students get great sequence data on the first or second try.

• System is flexible for many applications and has easy protocols.

• System can be used intermittently, common for educational settings.

Click here for more information on the LI-COR 4300 System.

Equipment and Reagents that may be needed for Template Preparation Instrumentation Qty Notes Incubator 1 Fisher # 11-690-525D Centrifuge 1 Fisher # 05-401-07 Refrigerator 1 - Freezer (-20, not frost-free) 1 Cost may be significantly higher if institutional regulations require flammable storage/explosion proof freezer Freezer (-80) 1 Fisher # 13-990-01 Agarose Gel Electrophoresis unit (with power supply) 1 Bio-Rad # 165-5004 Fluorometer or Spectrophotometer 1 Fisher # 11-600 Cloning vector 1 - Heat plate 1 Fisher # 11-497-1A Shaker Incubator 1 Cost will vary greatly depending on size Vortexer 2 Fisher # 12-810-1 Reagents Qty Notes Lysozyme 1 1g Fisher # ICN10083105 RNaseA 1 Sigma 250m STET Buffer 1 1L Fisher # BP24801 PEG/NaCl 1 500g PEG8,000 Fisher # AA4344336 Chloroform 1 500ml Ethanol 5 500ml 3M sodium acetate 1 - Isopropanol 1 1L TE Buffer 1 1L Growth Media 1 500g Agar 1 500g Agarose 1 25g Antibiotics 1 Cost is highly dependent of type of antibiotic used General Qty Notes 1.5 ml microcentrifuge tubes 1 Pk of 1000 Beakers 1 CS of 6x1L Bunson Burner 1 Fisher # 03-962 Ice - - Dry Ice - - Distilled, deionized water - - Pipet bulbs 8 Fisher # 13-681-50 Disposable Serological Pipets 3 1, 5, and 10 ml Culture Tubes 1 Cs of 500 Restriction Digests Qty Notes Restriction Enzymes 6 - Subcloning Qty Notes Competent Cells 1 20 transformations Clean-up kits (if desired) - User discretion Sequencing Qty Notes LI-COR 4300S DNA Analyzer 1 - Computer 1 - Thermal Cycler with Heated Lid 1 - General Qty Notes Micropipet (0.5-10ul, 10-100ul, 100-1000ul) 12 Number may vary according to number of students and group sizes (number given is for 4 groups) Micropipet tips 3 - PCR Tubes 1 Pk of 1000 0.20 mm Flat-tip loading tips 1 Pk of 1000 Reagents Qty Notes KBPlus Gel Matrix 5 - KBPlus TBE Buffer 5 - IRDye® infrared dye Labeled Primers 2 - IRDye® infrared dye Acycloterminators 4 - USB Thermosequenase Kits 12 - TEMED 1 25ml Ammonium Persulfate 1 25g Silane - -