How to Maximize Sensitivity on Chemiluminescent Western Blots ‑ Introduction
Listen to this introduction on "How to maximize the sensitivity of chemiluminescent Western blots".
Jeff and Jess discuss the choice of chemiluminescent substrates and the volume of substrate used and how these affect chemiluminescent Western blot sensitivity.
In the full version of this tips video, these additional topics are discussed:
- Placement of blot on film or imager
- Freshness of your Western blot (i.e., time since the substrate was added)
- Did the blot dry out before the chemiluminescent signal was captured?
- Sequence of imaging methods - which should be performed first after substrate addition?
- Length of acquisition time
- Temperature of chemiluminescent substrate when used
- Incubation time of blot with substrate
- Substrate concentration
Watch the full, 13-minute version of 'How to Maximize Sensitivity on Chemiluminescent Western Blots' now.
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