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Do You Know Your Leaf
Inside and Out?

Two Instruments – One Complete Picture

You know how complex a simple leaf is, but does your research reflect that knowledge? Two instruments can give you one complete picture, inside and out.
 

Get the Complete Picture


microsatellitesMicrosatellites

  • Completely automated from electrophoresis through allele scoring.
  • Unique allele “fingerprinting” software for highest scoring accuracy.
  • Accurately analyzes di-nucleotide repeats.
  • SagaGT software for automation, project management, allele scoring, and automatic data transfer to Oracle® database.
  • High throughput on 96-lane reloadable (3x) gels.

AFLPAFLP® Analysis

  • Optimized IRDye AFLP® and AFLP® expression kits.
  • Automates electrophoresis, band scoring, and binning alleles.
  • True gel images for accurate analysis and editing.
  • 192 lanes per run on reloadable gels.
  • Automatic data transfer to Oracle® database with flexible reporting.

TILLINGReverse Genetics – TILLING®

  • Observe partial loss of gene function in addition to knockouts.
  • High sensitivity and low background make it easy to detect mutation bands.
  • True gel images.
  • Two-color imaging eliminates false positive mutation identifications.
  • Screen up to 2 million bp per day.

SNP DiscoverySNP Discovery and Haplotyping – Ecotilling

  • High throughput, low cost technique for rapid discovery of polymorphisms in natural populations.
  • Screen up to 2300 samples or over 2 million base pairs per day.
  • Applications include mapping, association analysis, mutational profiling, and biodiversity.
  • Two color imaging eliminates false positives.
  • Costs a fraction of conventional resequencing approaches.
  • Rapid haplotyping – SNP discovery and haplotyping in a single step.

Long Read DNALong Read DNA Sequencing

  • 1000-1200 bases per sample.
  • 99% accuracy at 1000 bases.
  • Accurate for research and rugged for classroom demands.
  • Operates on demand without expensive start-up costs after inactivity.
  • On-board starter software for DNA sequencing, or comprehensive e-Seq autosequencing software for Windows®.

photosynthesisPhotosynthesis

  • Real-time measurement of carbon fixation (assimilation).
  • in vivo quantification of photosynthetic enzyme kinetics:
    • RUBISCO and PEP carboxylase – primary CO2 fixing enzymes.
    • RuBP regeneration – energy utilization in the dark reactions.
    • Temperature regulation of kinetic rates.
  • Assess quantum cost of each carbon captured (quantum efficiency).
  • Quantifies plasticity of photosynthetic machinery in response to shifting environmental conditions (light flecks, heat shock, water stress and others).

fluorescenceFluorescence

  • Rapid measurement of photon utilization for electron transport in leaves.
  • Calculate the quantum efficiency of electron transport in Photosystem II.
  • Evaluate energy lost to non-photosynthetic processes (non-photochemical quenching).

conductanceConductance

  • Monitor stomatal conductance to assess aperture regulation.
  • Measure CO2 transport across the plasma membrane (mesophyll conductance).
  • Track water consumption per carbon fixation or biomass gains though water use efficiency.

respirationRespiration

  • Mitochondrial respiration measured through CO2 release.
  • Temperature regulation of leaf sample to measure Q10.
  • Quantify soil CO2 flux from microbial and plant roots.

 

LI-6400XT

  • Innovative Designs: First photosynthesis measurement system with CO2 and H2O gas analyzers in the sensor head. The LI-6400 is the most referenced photosynthesis system in peer-reviewed scientific literature.

  • Integrated Measurements: First simultaneous gas exchange and fluorescence measurements over the same leaf area.

  • Chamber Variety: Interchangeable chamber configurations measure most sizes and shapes of leaves. Also supports user-built custom chambers.

  • Environmental Regulation: Software and hardware settings allow user-defined regulation of CO2, H2O, temperature and light.

  • Increased Productivity: Small chamber volumes and high flow rate allow for rapid leaf stabilization to steady state photosynthesis. Multiple sample facilitation through barcode reading.

  • Software and Graphing: Data analysis incorporated into software makes it possible to quickly monitor your results. Onboard diagnostic functions ensure high quality data and internet connectivity allows for remote monitoring and adjustment of the system.

4300

  • High Sensitivity: Infrared fluorescence detection is a key advantage due to low background at infrared wavelengths. Low background and LI-COR’s IRDye® reagents result in a wide dynamic detection range and very high quality data.

  • Independent Detection Channels: The 4300 System features two independent detection channels that use two separate lasers and detectors to eliminate errors due to fluorescence overlap. Fluorescence in each channel is separated by 100 nm.

  • Rugged and Reliable: The 4300 System is designed to meet demanding undergraduate training and research environments. Features such as diode lasers (similar to a CD player) make the instrument rugged and dramatically reduce maintenance and operation expenses. The instrument can also operate on demand after periods of inactivity without any expensive start-up costs.

  • Comprehensive Reagents: LI-COR’s wide ranging IRDye® reagent kits include everything from terminators for DNA sequencing, to kits for AFLP® expression analysis. All kits are optimized and quality controlled to get you started quickly and assure consistent results.
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4300: What's New

LEEF ProgramDNA Sequencing Introduction
Since their introduction more than 10 years ago, LI-COR infrared DNA sequencers have been characterized by very long reads with high accuracy. LI-COR's latest systems deliver 1000 - 1200 bp per sample (model 4300L) or 800 bp per sample (model 4300S) with 99% accuracy*.
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LEEF ProgramGenomics Solutions for Educators
LI-COR offers solutions and resources for administrators, educators, and students as well as matching funds programs. These programs represent part of LI-COR's ongoing commitment to helping train tomorrow's scientists and science teachers.
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LI-6400XT: What's New

LI-6400XT New LI-6400XT Available
See how the LI-6400 has been improved yet again. Faster 400 MHz processor, Ethernet connector, Open 6.0 software, and more improvements.
Go there View new features


LEEF Program LI-COR Environmental Education Fund (LEEF) Program
LI-COR is accepting applications for this year's LEEF II Program. The application deadline is December 31, 2007.
Go there Complete program information

   
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