AFLP® Expression Analysis Kits

  

LI-COR AFLP® Expression Analysis Kits are designed as a simple, cost-effective, and re-producible method to generate expression data using LI-COR automated DNA analyzers.

LI-COR's AFLP® Expression Analysis Kit is available with either IRDye® 800 infrared dye or IRDye® 700 infrared dye labels. Each kit is sufficient for 10 cDNA templates, with up to 64 expression profiles per template. Additional supplementary reagents for conducting genome-wide expression studies are also available.

image LI-COR's AFLP® Expression Analysis Kit offers a complete solution to identifying differentially expressed genes.

Features

  • IRDye® infrared dyes are safer than radioactive labels.
  • Easier, safer, and requires less labor than developing your own kits.
  • Image data are obtained in several hours instead
    of 2-4 days.
  • The sensitivity of LI-COR's DNA analyzer reduces costs through lower reagent usage and the elimination of labeling steps.

When used in combination with LI-COR's Odyssey® Infrared Imaging System, transcript-derived fragments can be recovered for further characterization. The sensitivity provided by LI-COR instruments, combined with the availability of optimized kits, eliminates radioactive labeling steps.

Ordering Information

Expression
Analysis Methods

"Biological responses and developmental processes are precisely controlled at the level of gene expression. Information on the temporal and spatial regulation of gene expression often sheds light on the potential function of a particular gene. Hence, an essential aspect of functional genomics is the transcriptome; i.e., the analysis of expression patterns of genes on a large scale.

There are currently three high-throughput techniques for large-scale monitoring of gene expression:

1. Serial analysis of gene expression (SAGE);

2. Hybridization-based methods;

3. Gel-based RNA fingerprinting tech- niques such as differential display and cDNA AFLP®.

In principle, SAGE can provide quantitative data concerning gene expression, but is expensive and labor intensive when multiple sample points are to be compared.

Microarray technology is extremely powerful in generating a broad view of gene expression. Unlike cDNA arrays, oligonucleotide arrays are able to distinguish between highly homologous sequences. However, the design of oligonucleotide arrays requires comprehensive sequence knowledge, at present only available for a small number of organisms.

cDNA AFLP® is an inexpensive, gel-based method for analysis of gene expression patterns and can be performed in any laboratory."

Ling Qin, et al.,1
 

Related Products

LI-COR's 4300 DNA Analysis System is ideal for AFLP® research. Data are generated quickly in a true gel format, allowing for accurate analysis. Two dyes are separated spectrally by 110 nm, eliminating crosstalk between channels and ensuring correct scoring. This reliable platform is economical to operate and maintain. A variety of configurations are available to accommodate AFLP® researchers' budgets.

The Odyssey™ Infrared Imaging System is used for band extractions and Northern confirmation analysis. Contact LI-COR for more information on these systems or any of our AFLP® kits, reagents, or software.

AFLP® Quantar™ Pro - Specialized software for co-dominant scoring of LI-COR AFLP® data. Contact Keygene Products for details.

1 Qin, Ling; Prins, Pjotr;, Jones, John T.; Popeijus, Herman; Smant, Geert; Bakker, Jaap; and Helder, Johannes. (2001) GenEST, A Powerful Bi-Directional Link Between cDNA Sequence Data and Gene Expression Profiles Generated by cDNA AFLP®. Nucleic Acids Research, Vol. 29, No. 7, 1616-1622. By permission of Oxford University Press.

AFLP® is a registered trademark of Keygene, N.V.

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