Efficient Mutation Screening

TILLING can be used as a high throughput screening technique. With the 4300 System as the enabling technology, the following TILLING performance results can be achieved with a single instrument*:

• Up to 750,000 base pairs screened per run.
• Up to 2000 samples screened per day.
• Up to 2 million base pairs screened per day.
• 1000 base pairs per sample.

In the lab, the 4300 System is fast and efficient:

• 96-well membrane combs load in just a few minutes.
• 2-4 hour run time (dependent on the size of the amplicon).
• Pre-mixed KB^Plus gel formulations.
• Gels can be reloaded several times.

Proven Performance, Impressive Results

The strength of the 4300 System for TILLING is being proven every day in multiple labs around the world. For example, in the first year of operation, the Arabidopsis TILLING Project (ATP) used LI-COR systems to deliver more than 1000 mutations in over 100 genes to Arabidopsis researchers (4).

Expansion When You Need It

With competition for research funds at an all time high, it is often necessary to demonstrate proof of concept prior to the acquisition of full funding. A single 4300 System with its combination of high productivity and moderate initial investment is ideal for early phase research. After full funding, the modular design allows unlimited expansion with no modification in experimental technique. A single technician can comfortably manage up to four instruments.