- After creating an AlignIR project and importing your files,
you can use the automatic alignment software to produce
a consensus that is ready for detailed examination.
-
The consensus can be calculated using either a weighted
or minimum method. The weighted method uses the base
letter with the highest count as the consensus call.
The minimum method takes all base letters into account
and generates an IUPAC ambiguity code for combinations
of bases at that position.
-
The consensus sequence is shown in a row below the
last sample sequence.
-
The Annotation row makes it easy to see ambiguities
and mismatches in the consensus sequence.
-
A variety of color schemes can be used in the AlignIR
window to highlight sequence differences.
|
