Quality Values Comparable
to PHRED

Each base call includes a quality value that gives the error probability for the call. e-Seq quality values are similar to those calculated by PHRED sequencing software. LI-COR sample files are compatible with PHRED software for base calling and quality value generation. Sequence files generated by e-Seq can also be imported directly into PHRAP for assembly.

Integrated Quality Values

Quality values are integrated into e-Seq operation. Edit windows show image, curve and quality values side-by-side, making it easy to examine low quality base calls. The average quality value of sequence data for a sample can be used as a threshold to determine whether a sample is included in assemblies or reports.

e-Seq's sample view window shows image, curve, and quality value data side-by-side. Green quality value bars are quality values above 40, and blue quality value bars represent quality values between 30 and 40. The numerical quality values can also be displayed instead of bars. Sequence data can be edited directly in the sample view window, making it easy to find and edit individual base calls that have low quality values.