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Template Preparation
|
Instrumentation
|
Qty
|
Notes
|
| Incubator |
1
|
Fisher # 11-690-525D |
| Centrifuge |
1
|
Fisher # 05-401-07 |
| Refrigerator |
1
|
- |
| Freezer (-20, not frost-free) |
1
|
Cost may be significantly higher if institutional regulations require flammable storage/explosion proof freezer |
| Freezer (-80) |
1
|
Fisher # 13-990-01 |
| Agarose Gel Electrophoresis unit (with power supply) |
1
|
Bio-Rad # 165-5004 |
| Fluorometer or Spectrophotometer |
1
|
Fisher # 11-600 |
| Cloning vector |
1
|
- |
| Heat plate |
1
|
Fisher # 11-497-1A |
| Shaker Incubator |
1
|
Cost will vary greatly depending on size |
| Vortexer |
2
|
Fisher # 12-810-1 |
|
Reagents
|
Qty
|
Notes
|
| Lysozyme |
1
|
1g Fisher # ICN10083105 |
| RNaseA |
1
|
Sigma 250m |
| STET Buffer |
1
|
1L Fisher # BP24801 |
| PEG/NaCl |
1
|
500g PEG8,000 Fisher # AA4344336 |
| Chloroform |
1
|
500ml |
| Ethanol |
5
|
500ml |
| 3M sodium acetate |
1
|
- |
| Isopropanol |
1
|
1L |
| TE Buffer |
1
|
1L |
| Growth Media |
1
|
500g |
| Agar |
1
|
500g |
| Agarose |
1
|
25g |
| Antibiotics |
1
|
Cost is highly dependent of type of antibiotic used |
|
General
|
Qty
|
Notes
|
| 1.5 ml microcentrifuge tubes |
1
|
Pk of 1000 |
| Beakers |
1
|
CS of 6x1L |
| Bunson Burner |
1
|
Fisher # 03-962 |
| Ice |
-
|
- |
| Dry Ice |
-
|
- |
| Distilled, deionized water |
-
|
- |
| Pipet bulbs |
8
|
Fisher # 13-681-50 |
| Disposable Serological Pipets |
3
|
1, 5, and 10 ml |
| Culture Tubes |
1
|
Cs of 500 |
|
Qty
|
Notes
|
| Restriction Enzymes |
6
|
- |
|
Qty
|
Notes
|
| Competent Cells |
1
|
20 transformations |
| Clean-up kits (if desired) |
-
|
User discretion |
|
Qty
|
Notes
|
| LI-COR 4300S DNA Analyzer |
1
|
- |
| Computer |
1
|
- |
| Thermal Cycler with Heated Lid |
1
|
- |
|
Qty
|
Notes
|
| Micropipet (0.5-10ul, 10-100ul, 100-1000ul) |
12
|
Number may vary according to number of students and group sizes (number given is for 4 groups) |
| Micropipet tips |
3
|
- |
| PCR Tubes |
1
|
Pk of 1000 |
| 0.20 mm Flat-tip loading tips |
1
|
Pk of 1000 |
|
Qty
|
Notes
|
| KBPlus Gel Matrix |
5
|
- |
| KBPlus TBE Buffer |
5
|
- |
| IRDye® infrared dye Labeled Primers |
2
|
- |
| IRDye® infrared dye Acycloterminators |
4
|
- |
| USB Thermosequenase Kits |
12
|
- |
| TEMED |
1
|
25ml |
| Ammonium Persulfate |
1
|
25g |
| Silane |
-
|
- |
|
