LAB SPOTLIGHT
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Dr. Fazlul Sarkar, an innovative researcher in the cancer field for over 30 years, is now in his 19th year at the Karmanos Cancer Center at the Wayne State Medical School. His research focus is on multiple human malignancies, a broad area where he has achieved great success.
Funded by four RO1s, two for pancreas cancers and two for prostate, he maintains a lab of 8-10 researchers, whom he refers to as the bread and butter of his lab. Through their dedicated research, they were first to show that natural chemopreventive agents are much more useful as an adjunct to standard therapeutics such as chemotherapy or radiation therapy, and they explored the molecular mechanism (NF kappa B assay, hedgehog, b-catenin, AKT signaling pathways) and conducted many clinical trials.
"...We would not have been able to do this without the Odyssey."
- Dr. Fazul SarkarCurrently, Dr. Sarkar and his lab are very busy in EMT, drug resistance, and cancer stem cells integrated with research using natural agents or synthetic derivatives. They have made a custom synthetic compound library with 20 compounds in each library. They screen these compounds, and they have two patent pending. One significant achievement in this area was their creation of a synthetic version of a natural compound that is very poorly bioavailable; their synthetic analog is now highly bioavailable.
Dr. Sarkar has been an Odyssey owner since 2001, and now has two Odysseys and a MousePOD. Before using the Odyssey, he had to use a lot of radioisotopes for electophoretic mobility shift assay (EMSA) for NF kappa B, their most frequently performed assay (2-3 people do it per week). When he first encountered the Odyssey at an AACR meeting, he was very excited to learn how he could do this assay without isotopes using near infrared fluorescent detection. Once he had the Odyssey in his lab, he discovered that the Odyssey actually gave better sensitivity for the EMSAs and he was able to switch to the infrared detection and eliminated the use of radioisotopes for these assays. Dr. Sarkar states, “we would not have been able to do this without the Odyssey.”
After the EMSAs were established on Odyssey, they switched most of their Westerns to near infrared fluorescent detection and began to do in vivo mouse imaging as well. In fact the lab has a second system with a MousePod in an animal facility for their in vivo imaging studies.
LINKS
For more information about Dr. Sarkar, visit the links below:
As a result of their research using the Odyssey, Dr. Sarkar and his lab have produced more than 20 publications in 2009 alone (see selected publication list below)! Dr. Sarkar’s heavy use of the Odyssey, and great achievements that have resulted, make him very deserving of the title “Odyssey Expert”.
PUBLICATIONS
Data that Sarkar’s lab created using the Odyssey have been published in Cancer Res, JBC, JCB, Cancer Lett, etc.
The following are some samples of articles using Odyssey:
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Li Y, Wang Z, Kong D, Murthy S, Dou QP, Sheng S, Reddy GP, Sarkar FH. Li Y, Wang Z, Kong D, Li R, Sarkar SH, Sarkar FH. Banerjee S, Kaseb AO, Wang Z, Kong D, Mohammad M, Padhye S, Sarkar FH, Mohammad RM. Banerjee S, Wang Z, Kong D, Sarkar FH. |
Li Y, Ahmed F, Ali S, Philip PA, Kucuk O, Sarkar FH. Ahmad A, Banerjee S, Wang Z, Kong D, Sarkar FH. Ali S, Varghese L, Pereira L, Tulunay-Ugur OE, Kucuk O, Carey TE, Wolf GT, Sarkar FH. Wang Z, Kong D, Banerjee S, Li Y, Adsay NV, Abbruzzese J, Sarkar FH. |
